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- PDB-2z9v: Crystal structure of pyridoxamine-pyruvate aminotransferase compl... -

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Basic information

Entry
Database: PDB / ID: 2z9v
TitleCrystal structure of pyridoxamine-pyruvate aminotransferase complexed with pyridoxamine
ComponentsAspartate aminotransferase
KeywordsTRANSFERASE / AMINOTRANSFERASE / PYRIDOXAMINE / PYRUVATE
Function / homology
Function and homology information


pyridoxamine-pyruvate transaminase / pyridoxamine-pyruvate transaminase activity / glycine biosynthetic process, by transamination of glyoxylate / L-serine-pyruvate transaminase activity / alanine-glyoxylate transaminase activity / transaminase activity / pyridoxal phosphate binding
Similarity search - Function
Serine-pyruvate aminotransferase/2-aminoethylphosphonate-pyruvate transaminase / Aminotransferase class V domain / Aminotransferase class-V / Aspartate Aminotransferase, domain 1 / Aspartate Aminotransferase, domain 1 / Aspartate Aminotransferase; domain 2 / Type I PLP-dependent aspartate aminotransferase-like (Major domain) / Pyridoxal phosphate-dependent transferase, small domain / Pyridoxal phosphate-dependent transferase, major domain / Pyridoxal phosphate-dependent transferase ...Serine-pyruvate aminotransferase/2-aminoethylphosphonate-pyruvate transaminase / Aminotransferase class V domain / Aminotransferase class-V / Aspartate Aminotransferase, domain 1 / Aspartate Aminotransferase, domain 1 / Aspartate Aminotransferase; domain 2 / Type I PLP-dependent aspartate aminotransferase-like (Major domain) / Pyridoxal phosphate-dependent transferase, small domain / Pyridoxal phosphate-dependent transferase, major domain / Pyridoxal phosphate-dependent transferase / Alpha-Beta Complex / 3-Layer(aba) Sandwich / Alpha Beta
Similarity search - Domain/homology
Chem-PXM / Pyridoxamine--pyruvate transaminase
Similarity search - Component
Biological speciesMesorhizobium loti (bacteria)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.7 Å
AuthorsYoshikane, Y. / Yokochi, N. / Yamasaki, M. / Mizutani, K. / Ohnishi, K. / Mikami, B. / Hayashi, H. / Yagi, T.
CitationJournal: J.Biol.Chem. / Year: 2008
Title: Crystal structure of pyridoxamine-pyruvate aminotransferase from Mesorhizobium loti MAFF303099
Authors: Yoshikane, Y. / Yokochi, N. / Yamasaki, M. / Mizutani, K. / Ohnishi, K. / Mikami, B. / Hayashi, H. / Yagi, T.
History
DepositionSep 26, 2007Deposition site: PDBJ / Processing site: PDBJ
Revision 1.0Nov 6, 2007Provider: repository / Type: Initial release
Revision 1.1Jul 13, 2011Group: Non-polymer description / Version format compliance
Revision 1.2Nov 1, 2023Group: Data collection / Database references ...Data collection / Database references / Derived calculations / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / struct_site
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _struct_site.pdbx_auth_asym_id / _struct_site.pdbx_auth_comp_id / _struct_site.pdbx_auth_seq_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: Aspartate aminotransferase
B: Aspartate aminotransferase
hetero molecules


Theoretical massNumber of molelcules
Total (without water)84,19213
Polymers83,0072
Non-polymers1,18511
Water13,565753
1
A: Aspartate aminotransferase
B: Aspartate aminotransferase
hetero molecules

A: Aspartate aminotransferase
B: Aspartate aminotransferase
hetero molecules


Theoretical massNumber of molelcules
Total (without water)168,38426
Polymers166,0144
Non-polymers2,37022
Water724
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
crystal symmetry operation7_555y,x,-z1
Buried area19220 Å2
ΔGint-229 kcal/mol
Surface area47560 Å2
MethodPISA
Unit cell
Length a, b, c (Å)68.628, 68.628, 312.191
Angle α, β, γ (deg.)90.00, 90.00, 90.00
Int Tables number96
Space group name H-MP43212

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Components

#1: Protein Aspartate aminotransferase / Pyridoxamine-pyruvate aminotransferase


Mass: 41503.512 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mesorhizobium loti (bacteria) / Strain: MAFF303099 / Gene: mlr6806 / Plasmid: PET21A / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3)
References: UniProt: Q988B8, pyridoxamine-pyruvate transaminase
#2: Chemical
ChemComp-SO4 / SULFATE ION


Mass: 96.063 Da / Num. of mol.: 5 / Source method: obtained synthetically / Formula: SO4
#3: Chemical ChemComp-PXM / 4-(AMINOMETHYL)-5-(HYDROXYMETHYL)-2-METHYLPYRIDIN-3-OL / PYRIDOXAMINE


Mass: 168.193 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C8H12N2O2
#4: Chemical
ChemComp-GOL / GLYCEROL / GLYCERIN / PROPANE-1,2,3-TRIOL


Mass: 92.094 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C3H8O3
#5: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 753 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.21 Å3/Da / Density % sol: 44.45 %
Crystal growTemperature: 277 K / Method: vapor diffusion, sitting drop / pH: 8.05
Details: 0.1M HEPES, 2M Ammonium sulfate, 10mM Pyridoxamine, pH 8.05, VAPOR DIFFUSION, SITTING DROP, temperature 277K

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: SPring-8 / Beamline: BL38B1 / Wavelength: 1 Å
DetectorType: RIGAKU RAXIS V / Detector: IMAGE PLATE / Date: Oct 27, 2005
RadiationMonochromator: DOUBLE-CRYSTAL MONOCHROMATOR / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1 Å / Relative weight: 1
ReflectionResolution: 1.7→50 Å / Num. obs: 82435 / % possible obs: 98.4 % / Observed criterion σ(I): -3 / Redundancy: 5.8 % / Biso Wilson estimate: 14.99 Å2 / Rmerge(I) obs: 0.039 / Net I/σ(I): 24.4
Reflection shellResolution: 1.7→1.76 Å / Redundancy: 3 % / Rmerge(I) obs: 0.113 / Num. unique all: 7561 / % possible all: 91.9

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Processing

Software
NameVersionClassification
REFMAC5.2.0019refinement
HKL-2000data collection
HKL-2000data reduction
HKL-2000data scaling
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: PDB ID 2Z9U

2z9u


Resolution: 1.7→14.94 Å / Cor.coef. Fo:Fc: 0.963 / Cor.coef. Fo:Fc free: 0.952 / SU B: 1.636 / SU ML: 0.056 / Cross valid method: THROUGHOUT / ESU R: 0.099 / ESU R Free: 0.096 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
RfactorNum. reflection% reflectionSelection details
Rfree0.18636 4094 5 %RANDOM
Rwork0.15559 ---
obs0.15714 78010 98.44 %-
Solvent computationIon probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK
Displacement parametersBiso mean: 14.852 Å2
Baniso -1Baniso -2Baniso -3
1-0 Å20 Å20 Å2
2--0 Å20 Å2
3---0 Å2
Refinement stepCycle: LAST / Resolution: 1.7→14.94 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms5832 0 73 753 6658
Refine LS restraints
Refine-IDTypeDev idealDev ideal targetNumber
X-RAY DIFFRACTIONr_bond_refined_d0.010.0226203
X-RAY DIFFRACTIONr_angle_refined_deg1.2481.9838486
X-RAY DIFFRACTIONr_chiral_restr0.0850.2953
X-RAY DIFFRACTIONr_gen_planes_refined0.0060.024711
X-RAY DIFFRACTIONr_nbd_refined0.1980.23252
X-RAY DIFFRACTIONr_nbtor_refined0.3080.24293
X-RAY DIFFRACTIONr_xyhbond_nbd_refined0.1160.2650
X-RAY DIFFRACTIONr_symmetry_vdw_refined0.1550.281
X-RAY DIFFRACTIONr_symmetry_hbond_refined0.430.232
X-RAY DIFFRACTIONr_mcbond_it0.561.54079
X-RAY DIFFRACTIONr_mcangle_it0.8926408
X-RAY DIFFRACTIONr_scbond_it1.75832425
X-RAY DIFFRACTIONr_scangle_it2.7724.52059
LS refinement shellResolution: 1.7→1.744 Å / Total num. of bins used: 20
RfactorNum. reflection% reflection
Rfree0.223 255 -
Rwork0.168 5183 -
obs-5183 90.54 %

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