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Open data
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Basic information
Entry | Database: PDB / ID: 2yn6 | ||||||
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Title | Pentameric Ligand-Gated Ion Channel ELIC in Complex with Barium | ||||||
![]() | PENTAMERIC LIGAND-GATED ION CHANNEL ELIC | ||||||
![]() | TRANSPORT PROTEIN / MEMBRANE PROTEIN / CATION SELECTIVE ION CHANNEL / PROKARYOTIC CYS-LOOP RECEPTOR | ||||||
Function / homology | ![]() extracellular ligand-gated monoatomic ion channel activity / regulation of membrane potential / transmembrane signaling receptor activity / neuron projection / signal transduction / identical protein binding / membrane Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Zimmermann, I. / Marabelli, A. / Bertozzi, C. / Sivilotti, L.G. / Dutzler, R. | ||||||
![]() | ![]() Title: Inhibition of the Prokaryotic Pentameric Ligand-Gated Ion Channel Elic by Divalent Cations. Authors: Zimmermann, I. / Marabelli, A. / Bertozzi, C. / Sivilotti, L.G. / Dutzler, R. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 314.7 KB | Display | ![]() |
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PDB format | ![]() | 257.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 476.4 KB | Display | ![]() |
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Full document | ![]() | 518.9 KB | Display | |
Data in XML | ![]() | 56.3 KB | Display | |
Data in CIF | ![]() | 74.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 2vl0S S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper:
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Components
#1: Protein | Mass: 36879.000 Da / Num. of mol.: 5 / Fragment: RESIDUES 22-343 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Chemical | ChemComp-BA / |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.6 Å3/Da / Density % sol: 65 % Description: STARTING MODEL 2VL0 WAS MISSING THE RESIDUE GLYCINE 164. CORRECTED IN THIS STRUCTURE |
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Crystal grow | pH: 6.5 / Details: 50 MM ADA PH6.5, 50 MM BAAC2, 10% PEG4000 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Mar 30, 2012 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.4 Å / Relative weight: 1 |
Reflection | Resolution: 3.3→30 Å / Num. obs: 38612 / % possible obs: 99.4 % / Observed criterion σ(I): 2 / Redundancy: 6.5 % / Biso Wilson estimate: 84.26 Å2 / Rmerge(I) obs: 0.12 / Net I/σ(I): 12.1 |
Reflection shell | Resolution: 3.3→3.5 Å / Redundancy: 6.1 % / Rmerge(I) obs: 0.64 / Mean I/σ(I) obs: 2.7 / % possible all: 96.6 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 2VL0 Resolution: 3.31→19.986 Å / SU ML: 0.45 / σ(F): 1.35 / Phase error: 27.73 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.31→19.986 Å
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Refine LS restraints |
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LS refinement shell |
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