A: REGULATOR OF RIBONUCLEASE ACTIVITY A B: REGULATOR OF RIBONUCLEASE ACTIVITY A C: REGULATOR OF RIBONUCLEASE ACTIVITY A D: REGULATOR OF RIBONUCLEASE ACTIVITY A E: REGULATOR OF RIBONUCLEASE ACTIVITY A F: REGULATOR OF RIBONUCLEASE ACTIVITY A G: REGULATOR OF RIBONUCLEASE ACTIVITY A H: REGULATOR OF RIBONUCLEASE ACTIVITY A I: REGULATOR OF RIBONUCLEASE ACTIVITY A J: REGULATOR OF RIBONUCLEASE ACTIVITY A K: REGULATOR OF RIBONUCLEASE ACTIVITY A L: REGULATOR OF RIBONUCLEASE ACTIVITY A M: ATP-DEPENDENT RNA HELICASE RHLB N: ATP-DEPENDENT RNA HELICASE RHLB
CHAINS M AND N ARE PEPTIDE OF SEQUENCE YRLTRPRTGNGPRRTGAPRNRRRSG WHICH CORRESPONDS TO RESIDUES 398- ...CHAINS M AND N ARE PEPTIDE OF SEQUENCE YRLTRPRTGNGPRRTGAPRNRRRSG WHICH CORRESPONDS TO RESIDUES 398-421 OF RHLB (UNIPROT P0A8J8) WITH AN ADDED N-TERMINAL TYROSINE RESIDUE, TO ENABLE ESTIMATION OF PEPTIDE CONCENTRATION BY UV ABSORPTION
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実験情報
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実験
実験
手法: X線回折 / 使用した結晶の数: 1
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試料調製
結晶
マシュー密度: 2.58 Å3/Da / 溶媒含有率: 52 % / 解説: NONE
結晶化
温度: 298 K / 手法: 蒸気拡散法, ハンギングドロップ法 / pH: 5.4 詳細: CRYSTALS WERE OBTAINED USING THE HANGING DROP METHOD, BY MIXING IN 1 TO 1 RATIO PROTEIN SAMPLE WITH MOTHER LIQUOR 100 MM SODIUM CITRATE PH 5.4, 32% MPD AND 200 MM AMMONIUM ACETATE AT 25 C. ...詳細: CRYSTALS WERE OBTAINED USING THE HANGING DROP METHOD, BY MIXING IN 1 TO 1 RATIO PROTEIN SAMPLE WITH MOTHER LIQUOR 100 MM SODIUM CITRATE PH 5.4, 32% MPD AND 200 MM AMMONIUM ACETATE AT 25 C. PLATES WERE THEN IMMEDIATELY TRANSFERRED TO 16 C. CRYSTALS WERE DIRECTLY FLASH FROZEN IN LIQUID NITROGEN.
解像度: 2.8→122.62 Å / Cor.coef. Fo:Fc: 0.935 / Cor.coef. Fo:Fc free: 0.888 / SU B: 19.997 / SU ML: 0.384 / 交差検証法: THROUGHOUT / ESU R Free: 0.456 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD / 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS.