CHAPERONE / SMALL HEAT SHOCK PROTEIN / STRESS PROTEIN / EYE LENS PROTEIN / CATARACT
Function / homology
Function and homology information
microtubule polymerization or depolymerization / negative regulation of intracellular transport / apoptotic process involved in morphogenesis / cardiac myofibril / regulation of programmed cell death / tubulin complex assembly / structural constituent of eye lens / negative regulation of amyloid fibril formation / M band / lens development in camera-type eye ...microtubule polymerization or depolymerization / negative regulation of intracellular transport / apoptotic process involved in morphogenesis / cardiac myofibril / regulation of programmed cell death / tubulin complex assembly / structural constituent of eye lens / negative regulation of amyloid fibril formation / M band / lens development in camera-type eye / muscle organ development / actin filament bundle / HSF1-dependent transactivation / negative regulation of reactive oxygen species metabolic process / negative regulation of protein-containing complex assembly / stress-activated MAPK cascade / synaptic membrane / muscle contraction / response to hydrogen peroxide / negative regulation of cell growth / cellular response to gamma radiation / Z disc / unfolded protein binding / protein folding / response to estradiol / amyloid-beta binding / response to heat / protein refolding / microtubule binding / perikaryon / dendritic spine / lysosome / protein stabilization / response to hypoxia / axon / negative regulation of gene expression / negative regulation of DNA-templated transcription / protein-containing complex binding / negative regulation of apoptotic process / structural molecule activity / cell surface / protein homodimerization activity / protein-containing complex / mitochondrion / extracellular exosome / nucleoplasm / identical protein binding / nucleus / metal ion binding / cytoplasm / cytosol Similarity search - Function
Alpha-crystallin B chain, ACD domain / Alpha-crystallin, N-terminal / Alpha crystallin A chain, N terminal / Alpha crystallin/Small heat shock protein, animal type / Immunoglobulin-like - #790 / Hsp20/alpha crystallin family / Small heat shock protein (sHSP) domain profile. / Alpha crystallin/Hsp20 domain / HSP20-like chaperone / Immunoglobulin-like ...Alpha-crystallin B chain, ACD domain / Alpha-crystallin, N-terminal / Alpha crystallin A chain, N terminal / Alpha crystallin/Small heat shock protein, animal type / Immunoglobulin-like - #790 / Hsp20/alpha crystallin family / Small heat shock protein (sHSP) domain profile. / Alpha crystallin/Hsp20 domain / HSP20-like chaperone / Immunoglobulin-like / Sandwich / Mainly Beta Similarity search - Domain/homology
Mass: 18.015 Da / Num. of mol.: 14 / Source method: isolated from a natural source / Formula: H2O
Compound details
ENGINEERED RESIDUE IN CHAIN A, ARG 120 TO HIS ENGINEERED RESIDUE IN CHAIN A, LEU 137 TO SER ...ENGINEERED RESIDUE IN CHAIN A, ARG 120 TO HIS ENGINEERED RESIDUE IN CHAIN A, LEU 137 TO SER ENGINEERED RESIDUE IN CHAIN B, ARG 120 TO HIS ENGINEERED RESIDUE IN CHAIN B, LEU 137 TO SER
Sequence details
R120G IS A DISEASE ASSOCIATED MUTATION L137M TO ALLOW CRYSTALISATION. ALPHA-CRYSTALLIN DOMAIN ONLY. ...R120G IS A DISEASE ASSOCIATED MUTATION L137M TO ALLOW CRYSTALISATION. ALPHA-CRYSTALLIN DOMAIN ONLY. HUMAN ALPHAB CRYSTALLIN ACD(RESIDUES 67-157)
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.49 Å3/Da / Density % sol: 50.6 % / Description: NONE
Crystal grow
pH: 7.4 Details: PROTEIN AT 16.5 MG/ML IN BUFFER (25 MM TRISHCL, PH 7.5, 25 MM NACL) WAS MIXED 1:1 WITH MOTHER LIQUOR (36% MPD, 0.18M POTASSIUM CHLORIDE)