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- PDB-2xwq: Anaerobic cobalt chelatase from Archeaoglobus fulgidus (CbiX) in ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2xwq | ||||||
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Title | Anaerobic cobalt chelatase from Archeaoglobus fulgidus (CbiX) in complex with metalated sirohydrochlorin product | ||||||
![]() | SIROHYDROCHLORIN COBALTOCHELATASE![]() | ||||||
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Function / homology | ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Ladakis, D. / Brindley, A.A. / Deery, E. / Warren, M.J. / Pickersgill, R.W. | ||||||
![]() | ![]() Title: Evolution in a Family of Chelatases Facilitated by the Introduction of Active Site Asymmetry and Protein Oligomerization. Authors: Romao, C.V. / Ladakis, D. / Lobo, S.A. / Carrondo, M.A. / Brindley, A.A. / Deery, E. / Matias, P.M. / Pickersgill, R.W. / Saraiva, L.M. / Warren, M.J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 121 KB | Display | ![]() |
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PDB format | ![]() | 96.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 2xvxC ![]() 2xvzC ![]() 2xwpC ![]() 2xwsC ![]() 2dj5S C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper:
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Components
#1: Protein | ![]() Mass: 15190.501 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() ![]() References: UniProt: O29537, ![]() #2: Chemical | #3: Water | ChemComp-HOH / | ![]() Nonpolymer details | COBALT-SIROHYDROCHLORIN (SIR): METAL AT THE CENTER OF THE SHC IS UNKNOWN, COBALT WAS USED FOR THE ...COBALT-SIROHYDROC | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.27 Å3/Da / Density % sol: 46 % / Description: NONE |
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Crystal grow![]() | pH: 6.8 / Details: pH 6.8 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC CCD / Detector: CCD / Date: Jan 26, 2010 / Details: MIRRORS |
Radiation | Monochromator: SILICON MONOCHROMATOR / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 2→50.3 Å / Num. obs: 253190 / % possible obs: 100 % / Observed criterion σ(I): 0 / Redundancy: 6.8 % / Biso Wilson estimate: 25.3 Å2 / Rmerge(I) obs: 0.15 / Net I/σ(I): 8.2 |
Reflection shell | Resolution: 2.01→2.12 Å / Redundancy: 7 % / Rmerge(I) obs: 0.62 / Mean I/σ(I) obs: 2.9 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: PDB ENTRY 2DJ5 Resolution: 2.01→45.57 Å / Cor.coef. Fo:Fc: 0.934 / Cor.coef. Fo:Fc free: 0.892 / SU B: 5.98 / SU ML: 0.163 / Cross valid method: THROUGHOUT / ESU R: 0.226 / ESU R Free: 0.206 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. U VALUES REFINED INDIVIDUALLY
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 31.913 Å2
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Refinement step | Cycle: LAST / Resolution: 2.01→45.57 Å
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Refine LS restraints |
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