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- PDB-2xpn: Crystal structure of a Spt6-Iws1(Spn1) complex from Encephalitozo... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2xpn | ||||||
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Title | Crystal structure of a Spt6-Iws1(Spn1) complex from Encephalitozoon cuniculi, Form I | ||||||
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![]() | TRANSCRIPTION / ELONGATION / HISTONE CHAPERONE / RNA POLYMERASE II / MRNA EXPORT | ||||||
Function / homology | ![]() nucleosome organization / transcription elongation-coupled chromatin remodeling / poly(A)+ mRNA export from nucleus / nucleosome binding / transcription elongation factor complex / histone binding / nucleus Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Diebold, M.-L. / Koch, M. / Cura, V. / Cavarelli, J. / Romier, C. | ||||||
![]() | ![]() Title: The Structure of an Iws1/Spt6 Complex Reveals an Interaction Domain Conserved in Tfiis, Elongin a and Med26 Authors: Diebold, M.-L. / Koch, M. / Loeliger, E. / Cura, V. / Winston, F. / Cavarelli, J. / Romier, C. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 83.5 KB | Display | ![]() |
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PDB format | ![]() | 62.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 433.4 KB | Display | ![]() |
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Full document | ![]() | 433.6 KB | Display | |
Data in XML | ![]() | 9.3 KB | Display | |
Data in CIF | ![]() | 12.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 2xplSC ![]() 2xpoC ![]() 2xppC C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 16424.105 Da / Num. of mol.: 1 / Fragment: CONSERVED DOMAIN, RESIDUES 55-198 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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#2: Protein/peptide | Mass: 2712.939 Da / Num. of mol.: 1 / Fragment: N-TERMINAL FRAGMENT, RESIDUES 53-71 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
#3: Chemical | ChemComp-BR / |
#4: Water | ChemComp-HOH / |
Sequence details | B 49-50 PART OF THROMBIN CLEAVAGE SITE. B 51-52 PART OF NDEI CLONING SITE. |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.71 Å3/Da / Density % sol: 55 % / Description: NONE |
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Crystal grow | Details: 22% PEG3350, 0.2 M NABR |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC CCD / Detector: CCD / Date: Nov 29, 2008 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9762 Å / Relative weight: 1 |
Reflection | Resolution: 1.95→50 Å / Num. obs: 16077 / % possible obs: 99.4 % / Observed criterion σ(I): 2 / Redundancy: 12.4 % / Rmerge(I) obs: 0.04 / Net I/σ(I): 78.8 |
Reflection shell | Resolution: 1.95→2.02 Å / Redundancy: 11.4 % / Rmerge(I) obs: 0.17 / Mean I/σ(I) obs: 17 / % possible all: 99.6 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 2XPL Resolution: 1.95→49.45 Å / Cor.coef. Fo:Fc: 0.939 / Cor.coef. Fo:Fc free: 0.931 / SU B: 7.239 / SU ML: 0.1 / Cross valid method: THROUGHOUT / ESU R: 0.159 / ESU R Free: 0.142 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. U VALUES WITH TLS ADDED. ATOM RECORD CONTAINS SUM OF TLS AND RESIDUAL B FACTORS.
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 31.327 Å2
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Refinement step | Cycle: LAST / Resolution: 1.95→49.45 Å
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Refine LS restraints |
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