SHEET THE SHEET STRUCTURE OF THIS MOLECULE IS BIFURCATED. IN ORDER TO REPRESENT THIS FEATURE IN ... SHEET THE SHEET STRUCTURE OF THIS MOLECULE IS BIFURCATED. IN ORDER TO REPRESENT THIS FEATURE IN THE SHEET RECORDS BELOW, TWO SHEETS ARE DEFINED.
ENGINEERED RESIDUE IN CHAIN A, TYR 132 TO PHE ENGINEERED RESIDUE IN CHAIN D, TYR 132 TO PHE
配列の詳細
TRANSPOSASE HAS Y132F MUTATION. DNA DERIVES FROM DEINOCOCCUS RADIODURANS IDRA2 TRANPOSON INTERNAL SEQUENCE
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実験情報
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実験
実験
手法: X線回折 / 使用した結晶の数: 1
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試料調製
結晶
マシュー密度: 2.68 Å3/Da / 溶媒含有率: 53.9 % / 解説: NONE
結晶化
温度: 292 K / pH: 4.8 詳細: COMPLEX BUFFER: 35MM TRIS-HCL PH 8.0 0.15M NACL, 10MM MGCL2, 0.5 MM TCEP. PRECIPITATING AGENT: 10MM CDCL2, 0.1M NA-ACETATE PH 4.8, 14% PEG4000. PROTEIN AT 9MG/ML IN COMPLEX BUFFER WAS MIXED ...詳細: COMPLEX BUFFER: 35MM TRIS-HCL PH 8.0 0.15M NACL, 10MM MGCL2, 0.5 MM TCEP. PRECIPITATING AGENT: 10MM CDCL2, 0.1M NA-ACETATE PH 4.8, 14% PEG4000. PROTEIN AT 9MG/ML IN COMPLEX BUFFER WAS MIXED WITH LE27 AND T5G. PROTEIN AND DNA OLIGONUCLEOTIDES WERE MIXED IN THE RATIO 1:1.1:1.3. EQUAL VOLUMES OF THIS COMPLEX AND PRECIPITATING AGENT WAS MIXED AT 19 C. CRYOPROTECTION IN 20% V/V GLYCEROL.
解像度: 1.9→30 Å / Rfactor Rfree error: 0.007 / Data cutoff high absF: 10000 / 交差検証法: THROUGHOUT / σ(F): 0 詳細: AS THE CRYSTALS WERE GROWN IN THE PRESENCE OF BOTH MG2+ AND CD2+, SOME METAL-ION BINDING SITES CONTAINED BOTH AT THE SAME POSITION WITH PARTIAL OCCUPANCIES. THIS WAS OBSERVED IN FO-FC ...詳細: AS THE CRYSTALS WERE GROWN IN THE PRESENCE OF BOTH MG2+ AND CD2+, SOME METAL-ION BINDING SITES CONTAINED BOTH AT THE SAME POSITION WITH PARTIAL OCCUPANCIES. THIS WAS OBSERVED IN FO-FC DIFFERENCE MAPS AND ANOMALOUS DIFFERENCE MAPS.