- PDB-2wsh: Structure of bacteriophage T4 EndoII E118A mutant -
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Open data
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Basic information
Entry
Database: PDB / ID: 2wsh
Title
Structure of bacteriophage T4 EndoII E118A mutant
Components
ENDONUCLEASE II
Keywords
HYDROLASE / GIY-YIG / NUCLEASE
Function / homology
Function and homology information
T4 deoxyribonuclease II / degradation of host chromosome by virus / symbiont-mediated suppression of host gene expression / endonuclease activity / DNA binding / metal ion binding Similarity search - Function
Mass: 18.015 Da / Num. of mol.: 373 / Source method: isolated from a natural source / Formula: H2O
Compound details
ENGINEERED RESIDUE IN CHAIN A, GLU 126 TO ALA ENGINEERED RESIDUE IN CHAIN B, GLU 126 TO ALA ...ENGINEERED RESIDUE IN CHAIN A, GLU 126 TO ALA ENGINEERED RESIDUE IN CHAIN B, GLU 126 TO ALA ENGINEERED RESIDUE IN CHAIN C, GLU 126 TO ALA ENGINEERED RESIDUE IN CHAIN D, GLU 126 TO ALA
Sequence details
THE SUBMITTED SEQUENCE IN THIS ENTRY IS 8 AMINO ACIDS SHORTER IN THE N-TERMINUS COMPARED TO OTHER ...THE SUBMITTED SEQUENCE IN THIS ENTRY IS 8 AMINO ACIDS SHORTER IN THE N-TERMINUS COMPARED TO OTHER DATABASE ENTRIES. THE SEQUENCE IS DESCRIBED IN CARLSON ET AL. (1999) , MOL. MICROBIOLOGY, 31(5)., P 1295
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.6 Å3/Da / Density % sol: 52.9 % Description: THE STARTING MODEL USED FOR MOLECULAR REPLACEMENT AS A PARTIAL MODEL OF ENDOII SOLVED PREVIOUSLY BY SAD.
Crystal grow
Temperature: 293 K / Method: microbatch / pH: 7 Details: CRYSTALS WERE GROWN AT 20 DEGREES, USING THE METHOD MICRO-BATCH UNDER OIL. 2.5 UL PROTEIN SOLUTION (3 MG/ML) WAS MIXED USING VORTEX WITH 1.75 OR 2 UL OF CRYSTALLIZATION SOLUTION CONTAINING ...Details: CRYSTALS WERE GROWN AT 20 DEGREES, USING THE METHOD MICRO-BATCH UNDER OIL. 2.5 UL PROTEIN SOLUTION (3 MG/ML) WAS MIXED USING VORTEX WITH 1.75 OR 2 UL OF CRYSTALLIZATION SOLUTION CONTAINING 30% PEG 3350, 100 MM BIS-TRIS BUFFER PH 7 AND 1% (W/V) N-OCTYL-B-D- GLUCOSIDE AND IMMEDIATELY PLACED UNDER PARAFFIN OIL. THE DROPS WERE IMMEDIATELY STREAK SEEDED AND CRYSTALS GREW IN A FEW HOURS.
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