- PDB-2wp0: Crystal structure of a HobA-DnaA (domain I-II) complex from Helic... -
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Basic information
Entry
Database: PDB / ID: 2wp0
Title
Crystal structure of a HobA-DnaA (domain I-II) complex from Helicobacter pylori.
Components
CHROMOSOMAL REPLICATION INITIATOR PROTEIN DNAA
HOBA
Keywords
DNA BINDING PROTEIN / NUCLEOTIDE-BINDING / DNA REPLICATION INITIATION / DIAA / HOBA / DNAA / ATP-BINDING
Function / homology
Function and homology information
regulation of DNA replication / DNA replication origin binding / DNA replication initiation / DNA replication / ATP binding / identical protein binding / metal ion binding / plasma membrane / cytoplasm Similarity search - Function
DNA replication regulator HobA / DNA replication regulator, HobA / DNA replication regulator HobA superfamily / DNA replication regulator / DnaA, N-terminal domain / DnaA, N-terminal domain superfamily / Chromosomal replication control, initiator DnaA / Chromosomal replication initiator, DnaA C-terminal / Chromosomal replication control, initiator DnaA, conserved site / Bacterial dnaA protein helix-turn-helix ...DNA replication regulator HobA / DNA replication regulator, HobA / DNA replication regulator HobA superfamily / DNA replication regulator / DnaA, N-terminal domain / DnaA, N-terminal domain superfamily / Chromosomal replication control, initiator DnaA / Chromosomal replication initiator, DnaA C-terminal / Chromosomal replication control, initiator DnaA, conserved site / Bacterial dnaA protein helix-turn-helix / DnaA protein signature. / Bacterial dnaA protein helix-turn-helix domain / Chromosomal replication control, initiator DnaA-like / Chromosomal replication initiator protein DnaA / Bacterial DnaA ATPAse domain / Trp repressor/replication initiator / GMP Synthetase; Chain A, domain 3 / ATPases associated with a variety of cellular activities / AAA+ ATPase domain / P-loop containing nucleoside triphosphate hydrolase / Rossmann fold / 2-Layer Sandwich / 3-Layer(aba) Sandwich / Alpha Beta Similarity search - Domain/homology
ACETATE ION / DNA replication regulator family protein / Chromosomal replication initiator protein DnaA Similarity search - Component
Mass: 18.015 Da / Num. of mol.: 107 / Source method: isolated from a natural source / Formula: H2O
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Details
Sequence details
THE ACCESSION NUMBER IS FOR THE FULL LENGTH PROTEIN
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.63 Å3/Da / Density % sol: 53.3 % Description: A COMBINATION OF 2UVP FOR HOBA AND A HOMOLGY MODEL FOR DNAA I-II WAS USED FOR MOLECULAR REPLACEMENT.
Crystal grow
pH: 8 Details: 100 MM TRIS-HCL PH 8.0, 200 MM K-ACETATE, 19-22% PEG 3350
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