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Open data
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Basic information
Entry | Database: PDB / ID: 2w3y | ||||||
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Title | Structure of the Evf virulence factor | ||||||
![]() | VIRULENCE FACTOR | ||||||
![]() | LIPID BINDING PROTEIN / DROSOPHILA PATHOGEN / ERWINIA CAROTOVORA / S-PALMITOYLATED PROTEIN / LIPID-BINDING PROTEIN | ||||||
Function / homology | Virulence factor Evf / Virulence factor Evf / PALMITIC ACID / Virulence factor![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Quevillon-Cheruel, S. / Leulliot, N. / Acosta Muniz C, C. / Vincent, M. / Gallay, J. / Argentini, M. / Cornu, D. / Boccard, F. / Lemaitre, B. / van Tilbeurgh, H. | ||||||
![]() | ![]() Title: Evf, a Virulence Factor Produced by the Drosophila Pathogen Erwinia Carotovora is a S-Palmitoylated Protein with a New Fold that Binds to Lipid Vesicles. Authors: Quevillon-Cheruel, S. / Leulliot, N. / Acosta Muniz, C. / Vincent, M. / Gallay, J. / Argentini, M. / Cornu, D. / Boccard, F. / Lemaitre, B. / Van Tilbeurgh, H. | ||||||
History |
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Remark 700 | SHEET THE SHEET STRUCTURE OF THIS MOLECULE IS BIFURCATED. IN ORDER TO REPRESENT THIS FEATURE IN ... SHEET THE SHEET STRUCTURE OF THIS MOLECULE IS BIFURCATED. IN ORDER TO REPRESENT THIS FEATURE IN THE SHEET RECORDS BELOW, TWO SHEETS ARE DEFINED. |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 118.5 KB | Display | ![]() |
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PDB format | ![]() | 97.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 32247.908 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Production host: ![]() ![]() #2: Chemical | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.12 Å3/Da / Density % sol: 41.59 % / Description: NONE |
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Crystal grow | Details: 25% PEG4000, 50MM BICINE PH 8 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC CCD / Detector: CCD |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9798 Å / Relative weight: 1 |
Reflection | Resolution: 2→80 Å / Num. obs: 38113 / % possible obs: 100 % / Observed criterion σ(I): 2 / Redundancy: 27.7 % / Rmerge(I) obs: 0.1 / Net I/σ(I): 25.6 |
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Processing
Software | Name: REFMAC / Version: 5.2.0005 / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Refinement | Method to determine structure: ![]() Starting model: NONE Resolution: 2→62.87 Å / Cor.coef. Fo:Fc: 0.951 / Cor.coef. Fo:Fc free: 0.934 / SU B: 4.721 / SU ML: 0.134 / Cross valid method: THROUGHOUT / ESU R: 0.209 / ESU R Free: 0.182 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS.
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 33.19 Å2
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Refinement step | Cycle: LAST / Resolution: 2→62.87 Å
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