Mass: 18.015 Da / Num. of mol.: 192 / Source method: isolated from a natural source / Formula: H2O
Sequence details
THERMOSTABLE MUTATED PROTEIN AN UNIPROT ID COULD NOT BE FOUND FOR THIS ENTRY, NEVERTHELESS THE ...THERMOSTABLE MUTATED PROTEIN AN UNIPROT ID COULD NOT BE FOUND FOR THIS ENTRY, NEVERTHELESS THE AUTHOR SUGGESTS THE FOLLOWING MUTATIONS HAVE BEEN INTRODUCED TO THIS ENTRY: S7P,T13F,N14H,Y18F,Q34L,S35E,S71T
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 1.89 Å3/Da / Density % sol: 35 % / Description: NONE
Crystal grow
pH: 5.1 Details: 55% (W/V) PEG 400, 0.15 M LITHIUM SULPHATE, 0.1 M TRI-SODIUM ACETATE (PH 5.1)
Resolution: 1.9→31.78 Å / Cor.coef. Fo:Fc: 0.957 / Cor.coef. Fo:Fc free: 0.935 / SU B: 2.705 / SU ML: 0.083 / Cross valid method: THROUGHOUT / ESU R: 0.17 / ESU R Free: 0.152 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS.
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.204
663
5.1 %
RANDOM
Rwork
0.15
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obs
0.153
12430
93 %
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Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK