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データを開く
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基本情報
登録情報 | データベース: PDB / ID: 2vt8 | ||||||
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タイトル | Structure of a conserved dimerisation domain within Fbox7 and PI31 | ||||||
![]() | PROTEASOME INHIBITOR PI31 SUBUNIT | ||||||
![]() | HYDROLASE INHIBITOR / POLYMORPHISM | ||||||
機能・相同性 | ![]() negative regulation of proteasomal protein catabolic process / proteasome core complex / Regulation of ornithine decarboxylase (ODC) / Cross-presentation of soluble exogenous antigens (endosomes) / Somitogenesis / endopeptidase inhibitor activity / proteasome binding / Regulation of activated PAK-2p34 by proteasome mediated degradation / Autodegradation of Cdh1 by Cdh1:APC/C / APC/C:Cdc20 mediated degradation of Securin ...negative regulation of proteasomal protein catabolic process / proteasome core complex / Regulation of ornithine decarboxylase (ODC) / Cross-presentation of soluble exogenous antigens (endosomes) / Somitogenesis / endopeptidase inhibitor activity / proteasome binding / Regulation of activated PAK-2p34 by proteasome mediated degradation / Autodegradation of Cdh1 by Cdh1:APC/C / APC/C:Cdc20 mediated degradation of Securin / Asymmetric localization of PCP proteins / SCF-beta-TrCP mediated degradation of Emi1 / NIK-->noncanonical NF-kB signaling / Ubiquitin-dependent degradation of Cyclin D / AUF1 (hnRNP D0) binds and destabilizes mRNA / TNFR2 non-canonical NF-kB pathway / Assembly of the pre-replicative complex / Vpu mediated degradation of CD4 / Degradation of DVL / Ubiquitin Mediated Degradation of Phosphorylated Cdc25A / Dectin-1 mediated noncanonical NF-kB signaling / Cdc20:Phospho-APC/C mediated degradation of Cyclin A / Hh mutants are degraded by ERAD / Degradation of AXIN / Degradation of GLI1 by the proteasome / Activation of NF-kappaB in B cells / Hedgehog ligand biogenesis / Defective CFTR causes cystic fibrosis / Negative regulation of NOTCH4 signaling / GSK3B and BTRC:CUL1-mediated-degradation of NFE2L2 / G2/M Checkpoints / Vif-mediated degradation of APOBEC3G / Autodegradation of the E3 ubiquitin ligase COP1 / Hedgehog 'on' state / Regulation of RUNX3 expression and activity / Degradation of GLI2 by the proteasome / GLI3 is processed to GLI3R by the proteasome / FBXL7 down-regulates AURKA during mitotic entry and in early mitosis / MAPK6/MAPK4 signaling / APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1 / Degradation of beta-catenin by the destruction complex / ABC-family proteins mediated transport / Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha / CDK-mediated phosphorylation and removal of Cdc6 / CLEC7A (Dectin-1) signaling / SCF(Skp2)-mediated degradation of p27/p21 / Regulation of expression of SLITs and ROBOs / FCERI mediated NF-kB activation / Regulation of PTEN stability and activity / Interleukin-1 signaling / Orc1 removal from chromatin / Regulation of RAS by GAPs / Separation of Sister Chromatids / Regulation of RUNX2 expression and activity / The role of GTSE1 in G2/M progression after G2 checkpoint / UCH proteinases / KEAP1-NFE2L2 pathway / Antigen processing: Ubiquitination & Proteasome degradation / Downstream TCR signaling / RUNX1 regulates transcription of genes involved in differentiation of HSCs / Neddylation / ER-Phagosome pathway / ubiquitin-dependent protein catabolic process / proteasome-mediated ubiquitin-dependent protein catabolic process / Ub-specific processing proteases / protein heterodimerization activity / perinuclear region of cytoplasm / endoplasmic reticulum / protein homodimerization activity / nucleoplasm / membrane / cytosol 類似検索 - 分子機能 | ||||||
生物種 | ![]() | ||||||
手法 | ![]() ![]() | ||||||
![]() | Kirk, R.J. / Murray-Rust, J. / Knowles, P.P. / Laman, H. / McDonald, N.Q. | ||||||
![]() | ![]() タイトル: Structure of a Conserved Dimerization Domain within the F-Box Protein Fbxo7 and the Pi31 Proteasome Inhibitor. 著者: Kirk, R.J. / Laman, H. / Knowles, P.P. / Murray-Rust, J. / Lomonosov, M. / Meziane, E.K. / McDonald, N.Q. | ||||||
履歴 |
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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ダウンロードとリンク
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ダウンロード
PDBx/mmCIF形式 | ![]() | 66.1 KB | 表示 | ![]() |
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PDB形式 | ![]() | 49.6 KB | 表示 | ![]() |
PDBx/mmJSON形式 | ![]() | ツリー表示 | ![]() | |
その他 | ![]() |
-検証レポート
文書・要旨 | ![]() | 429.2 KB | 表示 | ![]() |
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文書・詳細版 | ![]() | 432.9 KB | 表示 | |
XML形式データ | ![]() | 12.9 KB | 表示 | |
CIF形式データ | ![]() | 17 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
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リンク
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集合体
登録構造単位 | ![]()
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2 | ![]()
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単位格子 |
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非結晶学的対称性 (NCS) | NCS oper: (Code: given Matrix: (-0.9756, 0.03852, 0.2162), ベクター: |
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要素
#1: タンパク質 | 分子量: 17198.361 Da / 分子数: 2 / 断片: RESIDUES 1-151 / 変異: YES / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() #2: 水 | ChemComp-HOH / | 構成要素の詳細 | ENGINEERED | |
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-実験情報
-実験
実験 | 手法: ![]() |
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試料調製
結晶 | マシュー密度: 2.28 Å3/Da / 溶媒含有率: 46 % / 解説: NONE |
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結晶化 | pH: 8 / 詳細: 20% PEG 3350, 0.1 M AMMONIUM IODIDE, pH 8 |
-データ収集
回折 | 平均測定温度: 100 K |
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放射光源 | 由来: ![]() |
検出器 | タイプ: MARRESEARCH / 検出器: IMAGE PLATE / 日付: 2004年1月6日 / 詳細: MIRRORS |
放射 | モノクロメーター: YALE MIRRORS / プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray |
放射波長 | 波長: 1.5418 Å / 相対比: 1 |
反射 | 解像度: 2.6→28.1 Å / Num. obs: 9607 / % possible obs: 99.6 % / Observed criterion σ(I): 2 / 冗長度: 7.1 % / Rmerge(I) obs: 0.08 / Net I/σ(I): 20.1 |
反射 シェル | 解像度: 2.6→2.74 Å / 冗長度: 6.5 % / Rmerge(I) obs: 0.39 / Mean I/σ(I) obs: 4.1 / % possible all: 97.9 |
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解析
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精密化 | 構造決定の手法: ![]() 開始モデル: PART-REFINED MODEL OF A L7M SEMET DERIVATIVE OBTAINED BY SAD PHASING 解像度: 2.6→28.09 Å / Cor.coef. Fo:Fc: 0.941 / Cor.coef. Fo:Fc free: 0.913 / SU B: 11.888 / SU ML: 0.252 / 交差検証法: THROUGHOUT / ESU R: 1.262 / ESU R Free: 0.331 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD / 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS.
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溶媒の処理 | イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.2 Å / 溶媒モデル: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
原子変位パラメータ | Biso mean: 42.62 Å2
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精密化ステップ | サイクル: LAST / 解像度: 2.6→28.09 Å
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拘束条件 |
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