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- PDB-2vqz: Structure of the cap-binding domain of influenza virus polymerase... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2vqz | ||||||
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Title | Structure of the cap-binding domain of influenza virus polymerase subunit PB2 with bound m7GTP | ||||||
![]() | POLYMERASE BASIC PROTEIN 2 | ||||||
![]() | TRANSCRIPTION / RNA-DEPENDENT RNA POLYMERASE / PB2 SUBUNIT / INFLUENZA VIRUS / CAP-BINDING DOMAIN | ||||||
Function / homology | ![]() cap snatching / symbiont-mediated suppression of host mRNA transcription via inhibition of RNA polymerase II activity / host cell mitochondrion / 7-methylguanosine mRNA capping / symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of MAVS activity / virion component / viral RNA genome replication / RNA-dependent RNA polymerase activity / virus-mediated perturbation of host defense response / DNA-templated transcription ...cap snatching / symbiont-mediated suppression of host mRNA transcription via inhibition of RNA polymerase II activity / host cell mitochondrion / 7-methylguanosine mRNA capping / symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of MAVS activity / virion component / viral RNA genome replication / RNA-dependent RNA polymerase activity / virus-mediated perturbation of host defense response / DNA-templated transcription / host cell nucleus / RNA binding Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Guilligay, D. / Tarendeau, F. / Resa-Infante, P. / Coloma, R. / Crepin, T. / Sehr, P. / Lewis, J. / Ruigrok, R.W.H. / Ortin, J. / Hart, D.J. / Cusack, S. | ||||||
![]() | ![]() Title: The Structural Basis for CAP Binding by Influenza Virus Polymerase Subunit Pb2. Authors: Guilligay, D. / Tarendeau, F. / Resa-Infante, P. / Coloma, R. / Crepin, T. / Sehr, P. / Lewis, J. / Ruigrok, R.W.H. / Ortin, J. / Hart, D.J. / Cusack, S. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 344.1 KB | Display | ![]() |
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PDB format | ![]() | 295.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 2.2 MB | Display | ![]() |
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Full document | ![]() | 2.2 MB | Display | |
Data in XML | ![]() | 36.8 KB | Display | |
Data in CIF | ![]() | 48.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS domain:
NCS domain segments:
NCS oper:
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Components
#1: Protein | Mass: 19316.877 Da / Num. of mol.: 5 / Fragment: CAP-BINDING DOMAIN, RESIDUES 318-483 / Mutation: YES Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Chemical | ChemComp-MGT / #3: Water | ChemComp-HOH / | Compound details | ENGINEERED RESIDUE IN CHAIN A, ARG 389 TO LYS ENGINEERED RESIDUE IN CHAIN B, ARG 389 TO LYS ...ENGINEERED | Nonpolymer details | 7N-METHYL-8-HYDROGUANOSINE-5'-TRIPHOSPHATE (MGT): CO-CRYSTALLISED SELENOMETHIONINE (MSE): ...7N-METHYL-8-HYDROGUANO | Sequence details | MUTATION R389K IN OUR SEQUENCE COMPARED TO DATABASE. | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.51 Å3/Da / Density % sol: 51 % / Description: NONE |
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Crystal grow | pH: 4.6 Details: 1 MICROLITRE OF PROTEIN SOLUTION AT 11 MG PER ML IN 50 MM TRIS-HCL (PH 8.0), 200 MM NACL, 2 MM DTT, 5 MM M7GTP WITH AN EQUAL VOLUME OF A SOLUTION CONTAINING 0.1 M CITRIC ACID PH 4.6, 1.6-1.8 M SODIUM FORMATE. |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC CCD / Detector: CCD / Date: Jul 8, 2007 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.934 Å / Relative weight: 1 |
Reflection | Resolution: 2.3→30 Å / Num. obs: 40785 / % possible obs: 98.4 % / Observed criterion σ(I): 0 / Redundancy: 3.48 % / Rmerge(I) obs: 0.08 / Net I/σ(I): 8.59 |
Reflection shell | Resolution: 2.3→2.38 Å / Redundancy: 3.38 % / Rmerge(I) obs: 0.48 / Mean I/σ(I) obs: 1.79 / % possible all: 97.3 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: NONE Resolution: 2.3→29.53 Å / Cor.coef. Fo:Fc: 0.946 / Cor.coef. Fo:Fc free: 0.92 / SU B: 13.245 / SU ML: 0.16 / Cross valid method: THROUGHOUT / ESU R: 0.317 / ESU R Free: 0.227 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS.
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 32.47 Å2
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Refinement step | Cycle: LAST / Resolution: 2.3→29.53 Å
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Refine LS restraints |
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