- PDB-2vnd: The N69Q mutant of Vibrio cholerae endonuclease I -
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ID or keywords:
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Basic information
Entry
Database: PDB / ID: 2vnd
Title
The N69Q mutant of Vibrio cholerae endonuclease I
Components
ENDONUCLEASE I
Keywords
HYDROLASE / ENDONUCLEASE / ENDONUCLEASE I / SALT ADAPTATION / VIBRIO / NUCLEASE / KINETICS / SECRETED / THERMODYNAMIC STABILITY
Function / homology
Function and homology information
DNA nuclease activity / Hydrolases; Acting on ester bonds; Endodeoxyribonucleases producing 5'-phosphomonoesters / DNA catabolic process / endonuclease activity / extracellular region / metal ion binding Similarity search - Function
Resolution: 1.7→15 Å / Cor.coef. Fo:Fc: 0.947 / Cor.coef. Fo:Fc free: 0.91 / SU B: 2.636 / SU ML: 0.088 / Cross valid method: THROUGHOUT / ESU R: 0.129 / ESU R Free: 0.136 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS.
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.259
1131
5.1 %
RANDOM
Rwork
0.197
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obs
0.2
20905
99 %
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Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: MASK