+Open data
-Basic information
Entry | Database: PDB / ID: 2uuz | ||||||
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Title | Orthorhombic crystal form of GamS from bacteriophage lambda. | ||||||
Components | HOST-NUCLEASE INHIBITOR PROTEIN GAM | ||||||
Keywords | INHIBITOR / BACTERIOPHAGE LAMBDA / NUCLEASE INHIBITOR / RECBCD INHIBITOR / PUTATIVE DNA MIMIC | ||||||
Function / homology | Function and homology information symbiont-mediated evasion of DNA end degradation by host / deoxyribonuclease inhibitor activity / symbiont-mediated suppression of host innate immune response / virus-mediated perturbation of host defense response Similarity search - Function | ||||||
Biological species | BACTERIOPHAGE LAMBDA (virus) | ||||||
Method | X-RAY DIFFRACTION / MIRAS / Resolution: 2.3 Å | ||||||
Authors | Court, R.I. / Cook, N. / Saikrishnan, K. / Wigley, D.B. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 2007 Title: The Crystal Structure of Lambda-Gam Protein Suggests a Model for Recbcd Inhibition. Authors: Court, R.I. / Cook, N. / Saikrishnan, K. / Wigley, D.B. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2uuz.cif.gz | 48.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2uuz.ent.gz | 35.2 KB | Display | PDB format |
PDBx/mmJSON format | 2uuz.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2uuz_validation.pdf.gz | 257.9 KB | Display | wwPDB validaton report |
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Full document | 2uuz_full_validation.pdf.gz | 263.9 KB | Display | |
Data in XML | 2uuz_validation.xml.gz | 8.5 KB | Display | |
Data in CIF | 2uuz_validation.cif.gz | 11.8 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/uu/2uuz ftp://data.pdbj.org/pub/pdb/validation_reports/uu/2uuz | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 11733.002 Da / Num. of mol.: 2 / Fragment: RESIDUES 40-138 Source method: isolated from a genetically manipulated source Source: (gene. exp.) BACTERIOPHAGE LAMBDA (virus) / Plasmid: PKM574 / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): B834 (DE3) / References: UniProt: P03702 #2: Water | ChemComp-HOH / | Sequence details | CONSTRUCT STARTS AT RESIDUE 40 OF REPORTED SEQUENCE. | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 5 |
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-Sample preparation
Crystal | Density Matthews: 2.86 Å3/Da / Density % sol: 48.5 % |
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Crystal grow | pH: 8.5 Details: 100 MM TRIS/HCL, PH 8.5 32-34 % PEG 4000 700-850 MM LICL |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ROTATING ANODE / Type: RIGAKU RUH3R / Wavelength: 1.542 |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Details: MIRRORS |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.542 Å / Relative weight: 1 |
Reflection | Resolution: 2.3→32 Å / Num. obs: 19530 / % possible obs: 94.6 % / Observed criterion σ(I): 2 / Redundancy: 4.7 % / Rmerge(I) obs: 0.06 / Net I/σ(I): 7.3 |
Reflection shell | Resolution: 2.3→2.42 Å / Redundancy: 5 % / Rmerge(I) obs: 0.25 / Mean I/σ(I) obs: 3.1 / % possible all: 94.6 |
-Processing
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Refinement | Method to determine structure: MIRAS / Resolution: 2.3→32 Å / Data cutoff high absF: 10000 / Cross valid method: THROUGHOUT / σ(F): 2 Details: N-TERMINAL RESIDUES 40-48 OF CHAIN A AND 40-51 OF CHAIN B ARE DISORDERED. THE C-TERMINAL RESIDUE (VAL 138) IS DISORDERED IN BOTH CHAINS.
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Solvent computation | Bsol: 52.0685 Å2 / ksol: 0.347938 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters |
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Refinement step | Cycle: LAST / Resolution: 2.3→32 Å
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Refine LS restraints |
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