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データを開く
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基本情報
登録情報 | データベース: PDB / ID: 2sga | |||||||||
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タイトル | ELECTRON DENSITY CALCULATIONS AS AN EXTENSION OF PROTEIN STRUCTURE REFINEMENT. STREPTOMYCES GRISEUS PROTEASE AT 1.5 ANGSTROMS RESOLUTION | |||||||||
![]() | PROTEINASE A | |||||||||
![]() | HYDROLASE (SERINE PROTEINASE) | |||||||||
機能・相同性 | ![]() streptogrisin A / serine-type endopeptidase activity / proteolysis / extracellular region 類似検索 - 分子機能 | |||||||||
生物種 | ![]() | |||||||||
手法 | ![]() | |||||||||
![]() | James, M.N.G. / Sielecki, A.R. | |||||||||
![]() | ![]() タイトル: Electron density calculations as an extension of protein structure refinement. Streptomyces griseus protease A at 1.5 A resolution. 著者: Moult, J. / Sussman, F. / James, M.N. #1: ![]() タイトル: Structures of Product and Inhibitor Complexes of Streptomyces Griseus Protease a at 1.8 Angstroms Resolution. A Model for Serine Protease Catalysis 著者: James, M.N.G. / Sielecki, A.R. / Brayer, G.D. / Delbaere, L.T.J. / Bauer, C.-A. #2: ![]() タイトル: Protein Structure Refinement. Streptomyces Griseus Serine Protease a at 1.8 Angstroms Resolution 著者: Sielecki, A.R. / Hendrickson, W.A. / Broughton, C.G. / Delbaere, L.T.J. / Brayer, G.D. / James, M.N.G. #3: ![]() タイトル: Structure of the Complex Formed between the Bacterial-Produced Inhibitor Chymostatin and the Serine Enzyme Streptomyces Griseus Protease A 著者: Delbaere, L.T.J. / Brayer, G.D. #4: ![]() タイトル: Crystallographic and Kinetic Investigations of the Covalent Complex Formed by a Specific Tetrapeptide Aldehyde and the Serine Protease from Streptomyces Griseus 著者: Brayer, G.D. / Delbaere, L.T.J. / James, M.N.G. / Bauer, C.-A. / Thompson, R.C. #5: ![]() タイトル: Molecular Structure of Crystalline Streptomyces Griseus Protease a at 2.8 Angstroms Resolution. II. Molecular Conformation, Comparison with Alpha-Chymotrypsin and Active-Site Geometry 著者: Brayer, G.D. / Delbaere, L.T.J. / James, M.N.G. #6: ![]() タイトル: Molecular Structure of Crystalline Streptomyces Griseus Protease a at 2.8 Angstroms Resolution. I. Crystallization, Data Collection and Structural Analysis 著者: Brayer, G.D. / Delbaere, L.T.J. / James, M.N.G. #7: ![]() タイトル: Amino Acid Sequence Alignment of Bacterial and Mammalian Pancreatic Serine Proteases Based on Topological Equivalences 著者: James, M.N.G. / Delbaere, L.T.J. / Brayer, G.D. #8: ![]() タイトル: Tertiary Structural Differences between Microbial Serine Proteases and Pancreatic Serine Enzymes 著者: Delbaere, L.T.J. / Hutcheon, W.L.B. / James, M.N.G. / Thiessen, W.E. | |||||||||
履歴 |
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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ダウンロードとリンク
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ダウンロード
PDBx/mmCIF形式 | ![]() | 48.9 KB | 表示 | ![]() |
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PDB形式 | ![]() | 34.4 KB | 表示 | ![]() |
PDBx/mmJSON形式 | ![]() | ツリー表示 | ![]() | |
その他 | ![]() |
-検証レポート
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
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-関連構造データ
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リンク
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集合体
登録構造単位 | ![]()
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単位格子 |
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Atom site foot note | 1: THE SIDE CHAIN OF ARG 221 IS VERY DISORDERED. COORDINATES FOR THE ATOMS BEYOND CB HAVE BEEN OMITTED. 2: RESIDUE 99A IS A CIS-PROLINE. | ||||||||||||
Components on special symmetry positions |
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要素
#1: タンパク質 | 分子量: 18016.625 Da / 分子数: 1 / 由来タイプ: 組換発現 由来: (組換発現) ![]() 参照: UniProt: P00776 |
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#2: 水 | ChemComp-HOH / |
-実験情報
-実験
実験 | 手法: ![]() |
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試料調製
結晶 | マシュー密度: 2.31 Å3/Da / 溶媒含有率: 46.76 % | ||||||||||||||||||
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結晶化 | *PLUS pH: 4.1 / 手法: equilibrium dialysis | ||||||||||||||||||
溶液の組成 | *PLUS
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解析
ソフトウェア | 名称: PROLSQ / 分類: 精密化 | ||||||||||||
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精密化 | 解像度: 1.5→12 Å / Rfactor Rwork: 0.126 詳細: THE WATER MOLECULES HAVE BEEN NUMBERED TO REFLECT THEIR ACCURACY, WITH HOH 1 BEING THE MOST ACCURATE. SOLVENT 1, ALTHOUGH REFINED AS WATER (OXYGEN ATOM), HAS BEEN INTERPRETED AS A NA+ ION. ...詳細: THE WATER MOLECULES HAVE BEEN NUMBERED TO REFLECT THEIR ACCURACY, WITH HOH 1 BEING THE MOST ACCURATE. SOLVENT 1, ALTHOUGH REFINED AS WATER (OXYGEN ATOM), HAS BEEN INTERPRETED AS A NA+ ION. SEE REFERENCE 3 ABOVE FOR FURTHER DETAILS. | ||||||||||||
精密化ステップ | サイクル: LAST / 解像度: 1.5→12 Å
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精密化 | *PLUS 最高解像度: 1.5 Å / 最低解像度: 8 Å / Num. reflection obs: 19594 / σ(I): 2 / Rfactor obs: 0.121 | ||||||||||||
溶媒の処理 | *PLUS | ||||||||||||
原子変位パラメータ | *PLUS |