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Yorodumi- PDB-1sgc: THE 1.8 ANGSTROMS STRUCTURE OF THE COMPLEX BETWEEN CHYMOSTATIN AN... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1sgc | |||||||||
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Title | THE 1.8 ANGSTROMS STRUCTURE OF THE COMPLEX BETWEEN CHYMOSTATIN AND STREPTOMYCES GRISEUS PROTEASE A. A MODEL FOR SERINE PROTEASE CATALYTIC TETRAHEDRAL INTERMEDIATES | |||||||||
Components |
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Keywords | HYDROLASE/HYDROLASE INHIBITOR / HYDROLASE (SERINE PROTEINASE) / HYDROLASE-HYDROLASE INHIBITOR COMPLEX | |||||||||
Function / homology | Function and homology information streptogrisin A / serine-type endopeptidase activity / proteolysis / extracellular region Similarity search - Function | |||||||||
Biological species | Streptomyces griseus (bacteria) Streptomyces hygroscopicus (bacteria) MC521-C8 | |||||||||
Method | X-RAY DIFFRACTION / Resolution: 1.8 Å | |||||||||
Authors | Delbaere, L.T.J. / Brayer, G.D. | |||||||||
Citation | Journal: J.Mol.Biol. / Year: 1985 Title: The 1.8 A structure of the complex between chymostatin and Streptomyces griseus protease A. A model for serine protease catalytic tetrahedral intermediates. Authors: Delbaere, L.T. / Brayer, G.D. #1: Journal: J.Mol.Biol. / Year: 1980 Title: Structure of the Complex Formed between the Bacterial-Produced Inhibitor Chymostatin and the Serine Enzyme Streptomyces Griseus Protease A Authors: Delbaere, L.T.J. / Brayer, G.D. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1sgc.cif.gz | 52 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1sgc.ent.gz | 35.9 KB | Display | PDB format |
PDBx/mmJSON format | 1sgc.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1sgc_validation.pdf.gz | 381.1 KB | Display | wwPDB validaton report |
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Full document | 1sgc_full_validation.pdf.gz | 389 KB | Display | |
Data in XML | 1sgc_validation.xml.gz | 6.8 KB | Display | |
Data in CIF | 1sgc_validation.cif.gz | 10.8 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/sg/1sgc ftp://data.pdbj.org/pub/pdb/validation_reports/sg/1sgc | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Atom site foot note | 1: RESIDUE 99A IS A CIS-PROLINE. 2: THE SIDE CHAIN ATOMS OF ARG 221 BEYOND CB WERE NOT WELL DEFINED AND, THEREFORE, ARE NOT INCLUDED IN THIS ENTRY. | |||||||||||||||
Components on special symmetry positions |
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-Components
#1: Protein | Mass: 18016.625 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Streptomyces griseus (bacteria) / References: UniProt: P00776 |
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#2: Protein/peptide | |
#3: Water | ChemComp-HOH / |
Compound details | THE INHIBITOR CHYMOSTATIN A HAS LAST RESIDUE PHENYLALANINAL REPRESENTED WITH ALTERNATE ...THE INHIBITOR CHYMOSTATI |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.3 Å3/Da / Density % sol: 46.45 % |
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Crystal grow | *PLUS Method: other / Details: Delbaere, L.T.J., (1980) J. Mol. Biol., 139, 45. |
-Data collection
Radiation | Scattering type: x-ray |
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Radiation wavelength | Relative weight: 1 |
Reflection | *PLUS Highest resolution: 1.8 Å / Num. all: 15941 / Num. obs: 15116 / Rmerge(I) obs: 0.021 |
-Processing
Software | Name: PROLSQ / Classification: refinement | ||||||||||||
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Refinement | Rfactor Rwork: 0.123 / Highest resolution: 1.8 Å | ||||||||||||
Refinement step | Cycle: LAST / Highest resolution: 1.8 Å
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Refinement | *PLUS Num. reflection obs: 11755 / σ(I): 3 / Rfactor obs: 0.123 | ||||||||||||
Solvent computation | *PLUS | ||||||||||||
Displacement parameters | *PLUS Biso mean: 11.5 Å2 |