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- PDB-2sga: ELECTRON DENSITY CALCULATIONS AS AN EXTENSION OF PROTEIN STRUCTUR... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2sga | |||||||||
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Title | ELECTRON DENSITY CALCULATIONS AS AN EXTENSION OF PROTEIN STRUCTURE REFINEMENT. STREPTOMYCES GRISEUS PROTEASE AT 1.5 ANGSTROMS RESOLUTION | |||||||||
![]() | PROTEINASE A | |||||||||
![]() | HYDROLASE (SERINE PROTEINASE) | |||||||||
Function / homology | ![]() streptogrisin A / serine-type endopeptidase activity / proteolysis / extracellular region Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | ![]() | |||||||||
![]() | James, M.N.G. / Sielecki, A.R. | |||||||||
![]() | ![]() Title: Electron density calculations as an extension of protein structure refinement. Streptomyces griseus protease A at 1.5 A resolution. Authors: Moult, J. / Sussman, F. / James, M.N. #1: ![]() Title: Structures of Product and Inhibitor Complexes of Streptomyces Griseus Protease a at 1.8 Angstroms Resolution. A Model for Serine Protease Catalysis Authors: James, M.N.G. / Sielecki, A.R. / Brayer, G.D. / Delbaere, L.T.J. / Bauer, C.-A. #2: ![]() Title: Protein Structure Refinement. Streptomyces Griseus Serine Protease a at 1.8 Angstroms Resolution Authors: Sielecki, A.R. / Hendrickson, W.A. / Broughton, C.G. / Delbaere, L.T.J. / Brayer, G.D. / James, M.N.G. #3: ![]() Title: Structure of the Complex Formed between the Bacterial-Produced Inhibitor Chymostatin and the Serine Enzyme Streptomyces Griseus Protease A Authors: Delbaere, L.T.J. / Brayer, G.D. #4: ![]() Title: Crystallographic and Kinetic Investigations of the Covalent Complex Formed by a Specific Tetrapeptide Aldehyde and the Serine Protease from Streptomyces Griseus Authors: Brayer, G.D. / Delbaere, L.T.J. / James, M.N.G. / Bauer, C.-A. / Thompson, R.C. #5: ![]() Title: Molecular Structure of Crystalline Streptomyces Griseus Protease a at 2.8 Angstroms Resolution. II. Molecular Conformation, Comparison with Alpha-Chymotrypsin and Active-Site Geometry Authors: Brayer, G.D. / Delbaere, L.T.J. / James, M.N.G. #6: ![]() Title: Molecular Structure of Crystalline Streptomyces Griseus Protease a at 2.8 Angstroms Resolution. I. Crystallization, Data Collection and Structural Analysis Authors: Brayer, G.D. / Delbaere, L.T.J. / James, M.N.G. #7: ![]() Title: Amino Acid Sequence Alignment of Bacterial and Mammalian Pancreatic Serine Proteases Based on Topological Equivalences Authors: James, M.N.G. / Delbaere, L.T.J. / Brayer, G.D. #8: ![]() Title: Tertiary Structural Differences between Microbial Serine Proteases and Pancreatic Serine Enzymes Authors: Delbaere, L.T.J. / Hutcheon, W.L.B. / James, M.N.G. / Thiessen, W.E. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 48.9 KB | Display | ![]() |
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PDB format | ![]() | 34.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 359.9 KB | Display | ![]() |
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Full document | ![]() | 360.7 KB | Display | |
Data in XML | ![]() | 5.1 KB | Display | |
Data in CIF | ![]() | 8.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Atom site foot note | 1: THE SIDE CHAIN OF ARG 221 IS VERY DISORDERED. COORDINATES FOR THE ATOMS BEYOND CB HAVE BEEN OMITTED. 2: RESIDUE 99A IS A CIS-PROLINE. | ||||||||||||
Components on special symmetry positions |
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Components
#1: Protein | Mass: 18016.625 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.31 Å3/Da / Density % sol: 46.76 % | ||||||||||||||||||
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Crystal grow | *PLUS pH: 4.1 / Method: equilibrium dialysis | ||||||||||||||||||
Components of the solutions | *PLUS
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Processing
Software | Name: PROLSQ / Classification: refinement | ||||||||||||
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Refinement | Resolution: 1.5→12 Å / Rfactor Rwork: 0.126 Details: THE WATER MOLECULES HAVE BEEN NUMBERED TO REFLECT THEIR ACCURACY, WITH HOH 1 BEING THE MOST ACCURATE. SOLVENT 1, ALTHOUGH REFINED AS WATER (OXYGEN ATOM), HAS BEEN INTERPRETED AS A NA+ ION. ...Details: THE WATER MOLECULES HAVE BEEN NUMBERED TO REFLECT THEIR ACCURACY, WITH HOH 1 BEING THE MOST ACCURATE. SOLVENT 1, ALTHOUGH REFINED AS WATER (OXYGEN ATOM), HAS BEEN INTERPRETED AS A NA+ ION. SEE REFERENCE 3 ABOVE FOR FURTHER DETAILS. | ||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.5→12 Å
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Refinement | *PLUS Highest resolution: 1.5 Å / Lowest resolution: 8 Å / Num. reflection obs: 19594 / σ(I): 2 / Rfactor obs: 0.121 | ||||||||||||
Solvent computation | *PLUS | ||||||||||||
Displacement parameters | *PLUS |