+Open data
-Basic information
Entry | Database: PDB / ID: 2rpf | ||||||||||||||||||
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Title | RecO-bound ssDNA | ||||||||||||||||||
Components | DNA (5'-D(*Keywords | DNA / ssDNA / RecO / RecA | Function / homology | DNA | Function and homology information Method | SOLUTION NMR / simulated annealing | Authors | Masuda, T. / Ito, Y. / Shibata, T. / Mikawa, T. | Citation | Journal: J.Biol.Chem. / Year: 2009 | Title: A non-canonical DNA structure enables homologous recombination in various genetic systems Authors: Masuda, T. / Ito, Y. / Terada, T. / Shibata, T. / Mikawa, T. History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2rpf.cif.gz | 25.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2rpf.ent.gz | 18.6 KB | Display | PDB format |
PDBx/mmJSON format | 2rpf.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/rp/2rpf ftp://data.pdbj.org/pub/pdb/validation_reports/rp/2rpf | HTTPS FTP |
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-Related structure data
Related structure data | 2rpdC 2rpeC 2rphC C: citing same article (ref.) |
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Similar structure data | |
Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: DNA chain | Mass: 1190.830 Da / Num. of mol.: 1 / Source method: obtained synthetically / Details: Nucleotide synthesis |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR |
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NMR experiment | Type: 2D 1H-1H NOESY |
-Sample preparation
Details | Contents: 0.5mM DNA, 100% D2O / Solvent system: 100% D2O |
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Sample | Conc.: 0.5 mM / Component: DNA |
Sample conditions | Ionic strength: 150 / pH: 7.1 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
NMR spectrometer | Type: Bruker Avance / Manufacturer: Bruker / Model: AVANCE / Field strength: 600 MHz |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | |||||||||
NMR representative | Selection criteria: lowest energy | |||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 10 / Representative conformer: 1 |