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Open data
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Basic information
Entry | Database: PDB / ID: 2rpd | ||||||||||||||||||
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Title | Mhr1p-bound ssDNA | ||||||||||||||||||
![]() | DNA (5'-D(*![]() DNA / ssDNA / Mhr1 / RecA | Function / homology | DNA | ![]() Method | SOLUTION NMR / simulated annealing | ![]() Masuda, T. / Ito, Y. / Shibata, T. / Mikawa, T. | ![]() ![]() Title: A non-canonical DNA structure enables homologous recombination in various genetic systems Authors: Masuda, T. / Ito, Y. / Terada, T. / Shibata, T. / Mikawa, T. History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 26.4 KB | Display | ![]() |
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PDB format | ![]() | 17.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 295.8 KB | Display | ![]() |
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Full document | ![]() | 321.9 KB | Display | |
Data in XML | ![]() | 3.4 KB | Display | |
Data in CIF | ![]() | 4.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 |
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NMR ensembles |
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Components
#1: DNA chain | Mass: 1190.830 Da / Num. of mol.: 1 / Source method: obtained synthetically / Details: Nucleotide synthesis |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR |
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NMR experiment | Type: 2D 1H-1H NOESY |
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Sample preparation
Details | Contents: 0.5mM DNA, 100% D2O / Solvent system: 100% D2O |
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Sample | Conc.: 0.5 mM / Component: DNA |
Sample conditions | Ionic strength: 50 / pH: 7.1 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
NMR spectrometer | Type: Bruker DRX / Manufacturer: Bruker / Model: DRX / Field strength: 600 MHz |
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Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | |||||||||
NMR representative | Selection criteria: lowest energy | |||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 10 / Representative conformer: 1 |