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- PDB-2rnx: The Structural Basis for Site-Specific Lysine-Acetylated Histone ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2rnx | |||||||||
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Title | The Structural Basis for Site-Specific Lysine-Acetylated Histone Recognition by the Bromodomains of the HUman Transcriptional Co-Activators PCAF and CBP | |||||||||
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![]() | TRANSFERASE/NUCLEAR PROTEIN / Bromodomain / Histone / Acetyltransferase / Acyltransferase / Cell cycle / Host-virus interaction / Nucleus / Polymorphism / Transcription / Transcription regulation / Acetylation / Chromosomal protein / DNA damage / DNA repair / DNA-binding / Methylation / Nucleosome core / Phosphoprotein / TRANSFERASE-NUCLEAR PROTEIN COMPLEX | |||||||||
Function / homology | ![]() sexual sporulation resulting in formation of a cellular spore / regulation of protein ADP-ribosylation / negative regulation of rRNA processing / histone H3K9 acetyltransferase activity / diamine N-acetyltransferase / global genome nucleotide-excision repair / RNA polymerase I upstream activating factor complex / positive regulation of transcription from RNA polymerase II promoter by glucose / diamine N-acetyltransferase activity / negative regulation of centriole replication ...sexual sporulation resulting in formation of a cellular spore / regulation of protein ADP-ribosylation / negative regulation of rRNA processing / histone H3K9 acetyltransferase activity / diamine N-acetyltransferase / global genome nucleotide-excision repair / RNA polymerase I upstream activating factor complex / positive regulation of transcription from RNA polymerase II promoter by glucose / diamine N-acetyltransferase activity / negative regulation of centriole replication / positive regulation of attachment of mitotic spindle microtubules to kinetochore / Physiological factors / Condensation of Prophase Chromosomes / SIRT1 negatively regulates rRNA expression / peptidyl-lysine acetylation / Activated PKN1 stimulates transcription of AR (androgen receptor) regulated genes KLK2 and KLK3 / lysine N-acetyltransferase activity, acting on acetyl phosphate as donor / Assembly of the ORC complex at the origin of replication / YAP1- and WWTR1 (TAZ)-stimulated gene expression / positive regulation of fatty acid biosynthetic process / actomyosin / internal peptidyl-lysine acetylation / histone H3 acetyltransferase activity / cyclin-dependent protein serine/threonine kinase inhibitor activity / replication fork protection complex / N-terminal peptidyl-lysine acetylation / Oxidative Stress Induced Senescence / ATAC complex / I band / cellular response to parathyroid hormone stimulus / Regulation of gene expression in late stage (branching morphogenesis) pancreatic bud precursor cells / RUNX3 regulates NOTCH signaling / SAGA complex / limb development / Regulation of FOXO transcriptional activity by acetylation / NOTCH4 Intracellular Domain Regulates Transcription / A band / NOTCH3 Intracellular Domain Regulates Transcription / peptide-lysine-N-acetyltransferase activity / regulation of tubulin deacetylation / histone acetyltransferase binding / positive regulation of transcription by RNA polymerase I / protein acetylation / Notch-HLH transcription pathway / Formation of WDR5-containing histone-modifying complexes / Formation of paraxial mesoderm / regulation of cell division / RNA Polymerase I Promoter Escape / nucleolar large rRNA transcription by RNA polymerase I / RNA Polymerase I Transcription Initiation / Estrogen-dependent gene expression / rRNA transcription / regulation of RNA splicing / regulation of embryonic development / acetyltransferase activity / regulation of DNA repair / CENP-A containing nucleosome / histone acetyltransferase activity / histone acetyltransferase / positive regulation of gluconeogenesis / transcription initiation-coupled chromatin remodeling / positive regulation of glycolytic process / gluconeogenesis / transcription coregulator activity / RNA polymerase II transcription regulatory region sequence-specific DNA binding / RUNX1 regulates genes involved in megakaryocyte differentiation and platelet function / B-WICH complex positively regulates rRNA expression / NOTCH1 Intracellular Domain Regulates Transcription / Metalloprotease DUBs / mitotic spindle / memory / kinetochore / Pre-NOTCH Transcription and Translation / Constitutive Signaling by NOTCH1 PEST Domain Mutants / Constitutive Signaling by NOTCH1 HD+PEST Domain Mutants / positive regulation of neuron projection development / histone deacetylase binding / vasodilation / cellular response to insulin stimulus / structural constituent of chromatin / nucleosome / rhythmic process / chromatin organization / heart development / HATs acetylate histones / cellular response to oxidative stress / DNA-binding transcription factor binding / Estrogen-dependent gene expression / transcription coactivator activity / regulation of cell cycle / chromatin remodeling / cell cycle / protein heterodimerization activity / negative regulation of cell population proliferation / centrosome / chromatin binding / regulation of DNA-templated transcription / regulation of transcription by RNA polymerase II / protein kinase binding / positive regulation of DNA-templated transcription Similarity search - Function | |||||||||
Biological species | ![]() ![]() ![]() | |||||||||
Method | SOLUTION NMR / simulated annealing, torsion angle dynamics | |||||||||
![]() | Zeng, L. / Zhang, Q. / Gerona-Navarro, G. / Zhou, M.M. | |||||||||
![]() | ![]() Title: Structural Basis of Site-Specific Histone Recognition by the Bromodomains of Human Coactivators PCAF and CBP/p300 Authors: Zeng, L. / Zhang, Q. / Gerona-Navarro, G. / Moshkina, N. / Zhou, M.M. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 847.2 KB | Display | ![]() |
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PDB format | ![]() | 707.8 KB | Display | ![]() |
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-Validation report
Summary document | ![]() | 368.3 KB | Display | ![]() |
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Full document | ![]() | 627 KB | Display | |
Data in XML | ![]() | 107.3 KB | Display | |
Data in CIF | ![]() | 137.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: Protein | Mass: 14052.226 Da / Num. of mol.: 1 / Fragment: UNP residues 719-832 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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#2: Protein/peptide | Mass: 1506.771 Da / Num. of mol.: 1 / Fragment: UNP residues 32-43 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Production host: ![]() ![]() |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR |
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NMR experiment | Type: 3D 1H-13C NOESY |
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Sample preparation
Details | Contents: 0.5 mM [U-100% 13C; U-100% 15N] potassium phosphate, 100% D2O Solvent system: 100% D2O |
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Sample | Conc.: 0.5 mM / Component: potassium phosphate / Isotopic labeling: [U-100% 13C; U-100% 15N] |
Sample conditions | Ionic strength: 0.1 / pH: 6.5 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
NMR spectrometer |
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Processing
NMR software |
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Refinement | Method: simulated annealing, torsion angle dynamics / Software ordinal: 1 | ||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 200 / Conformers submitted total number: 20 |