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- PDB-2r1s: Structure of the RNA brominated tridecamer r(GCGUU-5BUGAAACGC) at... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2r1s | ||||||||||||||||||
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Title | Structure of the RNA brominated tridecamer r(GCGUU-5BUGAAACGC) at 1.4 A (Br1) | ||||||||||||||||||
![]() | RNA (5'-R(*![]() RNA / asymmetry / electrostatic / monovalent cations / structural plasticity | Function / homology | RNA / RNA (> 10) | ![]() Method | ![]() ![]() ![]() Timsit, Y. / Bombard, S. | ![]() ![]() Title: The 1.3 A resolution structure of the RNA tridecamer r(GCGUUUGAAACGC): Metal ion binding correlates with base unstacking and groove contraction. Authors: Timsit, Y. / Bombard, S. History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 30.6 KB | Display | ![]() |
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PDB format | ![]() | 19.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 388.5 KB | Display | ![]() |
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Full document | ![]() | 388.5 KB | Display | |
Data in XML | ![]() | 5.6 KB | Display | |
Data in CIF | ![]() | 7.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: RNA chain | Mass: 4236.421 Da / Num. of mol.: 2 / Source method: obtained synthetically #2: Chemical | ChemComp-NA / #3: Chemical | ChemComp-MG / | #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.13 Å3/Da / Density % sol: 42.27 % | ||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 298 K / Method: vapor diffusion / pH: 7 Details: 30% MPD, 100mM NaCl, 50 mM Tris, 3mM MnCl2, 2mM MgCl2, 0.5 mM RNA, pH 7.0, VAPOR DIFFUSION, temperature 298K | ||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions |
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-Data collection
Diffraction | Mean temperature: 80 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MAR scanner 345 mm plate / Detector: IMAGE PLATE / Date: Feb 3, 2003 |
Radiation | Monochromator: Si (111) crystals / Protocol: MAD / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.919487 Å / Relative weight: 1 |
Reflection | Resolution: 1.4→14.1 Å / Num. obs: 12174 / % possible obs: 91 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 3.3 % / Rmerge(I) obs: 0.035 / Rsym value: 2.3 / Net I/σ(I): 19.4 |
Reflection shell | Resolution: 1.4→1.49 Å / Redundancy: 1.8 % / Rmerge(I) obs: 0.113 / Mean I/σ(I) obs: 8.8 / Num. unique all: 1310 / Rsym value: 8 / % possible all: 67.2 |
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Processing
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Refinement | Resolution: 1.4→14.1 Å / σ(F): 209 / Stereochemistry target values: mlf
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Solvent computation | Bsol: 47.016 Å2 | ||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 11.026 Å2
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Refinement step | Cycle: LAST / Resolution: 1.4→14.1 Å
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Refine LS restraints |
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Xplor file |
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