[English] 日本語
![](img/lk-miru.gif)
- PDB-2r22: Structure of the native RNA tridecamer r(GCGUUUGAAACGC) at 1.5 A ... -
+
Open data
-
Basic information
Entry | Database: PDB / ID: 2r22 | ||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Title | Structure of the native RNA tridecamer r(GCGUUUGAAACGC) at 1.5 A (NatMn) | ||||||||||||||||||
![]() | RNA (5'-R(*![]() RNA / monovalent / electrostatic / asymmetry / manganese | Function / homology | : / RNA / RNA (> 10) | ![]() Method | ![]() ![]() ![]() Timsit, Y. / Bombard, S. | ![]() ![]() Title: The 1.3 A structure of the tridecamer r(GCGUUUGAAACGC) Authors: Timsit, Y. / Bombard, S. History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 27.9 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 18.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
---|
-Related structure data
Related structure data | |
---|---|
Similar structure data |
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
|
-
Components
#1: RNA chain | Mass: 4157.525 Da / Num. of mol.: 2 / Source method: obtained synthetically #2: Chemical | ChemComp-NA / #3: Chemical | ChemComp-MN / | #4: Water | ChemComp-HOH / | |
---|
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 2.11 Å3/Da / Density % sol: 41.58 % | ||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Crystal grow | Method: vapor diffusion, hanging drop / pH: 7 Details: 3 mM MnCl2, 100 mM NaCl, 50 mM Tris, 30 % MPD, pH 7.0, VAPOR DIFFUSION, HANGING DROP | ||||||||||||||||||||||||||||||||||||
Components of the solutions |
|
-Data collection
Diffraction | Mean temperature: 80 K |
---|---|
Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MAR scanner 345 mm plate / Detector: IMAGE PLATE / Date: Feb 3, 2003 / Details: mirrors |
Radiation | Monochromator: Si (111) Crystal / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.919487 Å / Relative weight: 1 |
Reflection | Resolution: 1.4→20 Å / Num. all: 12814 / Num. obs: 12814 / % possible obs: 88.7 % / Redundancy: 1.7 % / Rmerge(I) obs: 0.09 / Rsym value: 6.4 / Net I/σ(I): 7.8 |
Reflection shell | Resolution: 1.4→1.53 Å / Redundancy: 1.1 % / Rmerge(I) obs: 0.279 / Mean I/σ(I) obs: 2.1 / Num. unique all: 1838 / Rsym value: 19.7 / % possible all: 92 |
-
Processing
Software |
| ||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Resolution: 1.4→20 Å / σ(F): 104
| ||||||||||||||||||||||||||||
Solvent computation | Bsol: 40.159 Å2 | ||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 13.727 Å2
| ||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.4→20 Å
| ||||||||||||||||||||||||||||
Refine LS restraints |
| ||||||||||||||||||||||||||||
Xplor file |
|