+Open data
-Basic information
Entry | Database: PDB / ID: 2qlh | ||||||
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Title | mPlum I65L mutant | ||||||
Components | Fluorescent protein plum | ||||||
Keywords | FLUORESCENT PROTEIN / far-red fluorescent protein / acylimine | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Discosoma sp. LW-2004 (sea anemone) | ||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 1.9 Å | ||||||
Authors | Shu, X. / Remington, S.J. | ||||||
Citation | Journal: To be Published Title: Structural Studies of Far-Red Emission in mPlum, a Monomeric Red Fluorescent Protein Authors: Shu, X. / Wang, L. / Colip, L. / Kallio, K. / Remington, S.J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2qlh.cif.gz | 107.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2qlh.ent.gz | 82.3 KB | Display | PDB format |
PDBx/mmJSON format | 2qlh.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2qlh_validation.pdf.gz | 452.1 KB | Display | wwPDB validaton report |
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Full document | 2qlh_full_validation.pdf.gz | 464.1 KB | Display | |
Data in XML | 2qlh_validation.xml.gz | 25.3 KB | Display | |
Data in CIF | 2qlh_validation.cif.gz | 36.9 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ql/2qlh ftp://data.pdbj.org/pub/pdb/validation_reports/ql/2qlh | HTTPS FTP |
-Related structure data
Related structure data | 2qlgC 2qliC 1g7kS C: citing same article (ref.) S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 25604.912 Da / Num. of mol.: 2 / Mutation: I66L Source method: isolated from a genetically manipulated source Source: (gene. exp.) Discosoma sp. LW-2004 (sea anemone) / Plasmid: pBAD / Production host: Escherichia coli (E. coli) / Strain (production host): TOP10 / References: UniProt: Q5S3G7 #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.23 Å3/Da / Density % sol: 44.76 % |
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Crystal grow | pH: 8.5 / Details: 200mM NaCl, 100mM Tris pH8.5, 30% PEG 3400 |
-Data collection
Diffraction source | Source: ROTATING ANODE / Type: RIGAKU / Wavelength: 1.54 Å |
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Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.54 Å / Relative weight: 1 |
Reflection | Resolution: 1.9→10 Å / Num. obs: 36504 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB entry 1G7K Resolution: 1.9→10 Å
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Refinement step | Cycle: LAST / Resolution: 1.9→10 Å
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