温度: 298 K / 手法: 蒸気拡散法, シッティングドロップ法 / pH: 5.5 詳細: The WT-TTR was concentrated to 4 mg/mL in 10 mM NaPi, 100 mM KCl, at pH 7.6 and co-crystallized at room temperature with inhibitors using the vapor-diffusion sitting drop method. Crystals ...詳細: The WT-TTR was concentrated to 4 mg/mL in 10 mM NaPi, 100 mM KCl, at pH 7.6 and co-crystallized at room temperature with inhibitors using the vapor-diffusion sitting drop method. Crystals were grown from 1.395 M sodium citrate, 3.5% v/v glycerol at pH 5.5. The crystals were frozen using a cryo-protectant solution of 1.395 M sodium citrate, pH 5.5, containing 10% v/v glycerol., VAPOR DIFFUSION, SITTING DROP, temperature 298.0K
タイプ: MARMOSAIC 325 mm CCD / 検出器: CCD / 日付: 2007年1月11日 詳細: Flat mirror (vertical focusing); single crystal Si(111) bent monochromator (ho rizontal focusing)
放射
プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray
放射波長
波長: 0.9791 Å / 相対比: 1
Reflection
冗長度: 6.9 % / Av σ(I) over netI: 11.8 / 数: 294859 / Rmerge(I) obs: 0.04 / Χ2: 1 / D res high: 1.45 Å / D res low: 50 Å / Num. obs: 42964 / % possible obs: 99.9
Diffraction reflection shell
最高解像度 (Å)
最低解像度 (Å)
% possible obs (%)
ID
Rmerge(I) obs
Chi squared
Redundancy
3.12
50
99.2
1
0.018
1.05
6.6
2.48
3.12
100
1
0.026
0.922
6.9
2.17
2.48
100
1
0.038
0.981
6.9
1.97
2.17
100
1
0.052
0.9
7
1.83
1.97
100
1
0.084
0.979
6.9
1.72
1.83
100
1
0.15
1.007
6.9
1.63
1.72
100
1
0.223
1.017
6.8
1.56
1.63
100
1
0.285
1.036
7
1.5
1.56
100
1
0.415
1.035
6.8
1.45
1.5
100
1
0.567
1.047
6.8
反射
解像度: 1.45→50 Å / Num. obs: 42964 / % possible obs: 99.9 % / 冗長度: 6.9 % / Biso Wilson estimate: 16.6 Å2 / Rsym value: 0.04 / Χ2: 0.997 / Net I/σ(I): 11.8
反射 シェル
解像度: 1.45→1.5 Å / 冗長度: 6.8 % / Mean I/σ(I) obs: 3.4 / Num. unique all: 4242 / Rsym value: 0.567 / Χ2: 1.047 / % possible all: 100