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- PDB-2pzl: Crystal structure of the Bordetella bronchiseptica enzyme WbmG in... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2pzl | ||||||
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Title | Crystal structure of the Bordetella bronchiseptica enzyme WbmG in complex with NAD and UDP | ||||||
![]() | Putative nucleotide sugar epimerase/ dehydratase | ||||||
![]() | SUGAR BINDING PROTEIN / ROSSMANN FOLD / PROTEIN-NAD COMPLEX / PROTEIN-NUCLEOTIDE COMPLEX | ||||||
Function / homology | ![]() UDP-L-rhamnose synthase activity / UDP-glucose 4,6-dehydratase activity / UDP-rhamnose biosynthetic process / dTDP-glucose 4,6-dehydratase activity / flavonol biosynthetic process / glutamine metabolic process / nucleotide binding Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | King, J.D. / Harmer, N.J. / Maskell, D.J. / Blundell, T.L. | ||||||
![]() | ![]() Title: Predicting protein function from structure--the roles of short-chain dehydrogenase/reductase enzymes in Bordetella O-antigen biosynthesis. Authors: King, J.D. / Harmer, N.J. / Preston, A. / Palmer, C.M. / Rejzek, M. / Field, R.A. / Blundell, T.L. / Maskell, D.J. #1: Journal: Acta Crystallogr.,Sect.F / Year: 2007 Title: Cloning, expression, purification and preliminary crystallographic analysis of the short-chain dehydrogenase enzymes WbmF, WbmG and WbmH from Bordetella bronchiseptica. Authors: Harmer, N.J. / King, J.D. / Palmer, C.M. / Preston, A. / Maskell, D.J. / Blundell, T.L. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 135.2 KB | Display | ![]() |
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PDB format | ![]() | 104.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.6 MB | Display | ![]() |
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Full document | ![]() | 1.6 MB | Display | |
Data in XML | ![]() | 26 KB | Display | |
Data in CIF | ![]() | 35.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 2pzjC ![]() 2pzkSC ![]() 2pzmC ![]() 2q1sC ![]() 2q1tC ![]() 2q1uC ![]() 2q1wC S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS domain:
NCS domain segments: Component-ID: 1 / Ens-ID: 1 / Beg auth comp-ID: SER / Beg label comp-ID: SER / End auth comp-ID: SER / End label comp-ID: SER / Refine code: 5 / Auth seq-ID: -1 - 306 / Label seq-ID: 19 - 326
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Details | The biological unit is a dimer, formed from chains A and B. |
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Components
#1: Protein | Mass: 35287.984 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Chemical | #3: Chemical | #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.51 Å3/Da / Density % sol: 51.01 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 8.5 Details: 0.1 M Tris, 0.2 M MgCl2, 16-18 % (w/w) PEG 8000. UDP-glucose was added by soaking at 10 mM prior to cryoprotection. pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Dec 13, 2004 |
Radiation | Monochromator: Si 111 Channel / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.488 Å / Relative weight: 1 |
Reflection | Resolution: 2.39→30 Å / Num. all: 27912 / Num. obs: 27549 / % possible obs: 98.7 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 4 / Redundancy: 4.47 % / Rmerge(I) obs: 0.103 / Net I/σ(I): 15 |
Reflection shell | Resolution: 2.4→2.46 Å / Redundancy: 4.45 % / Rmerge(I) obs: 0.328 / Mean I/σ(I) obs: 3.98 / % possible all: 97.7 |
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Processing
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Refinement | Method to determine structure: Single Wavelength Starting model: RELATED PDB ENTRY 2PZK WAS SOLVED BY MAD, AND USED AS A STARTING MODEL. Resolution: 2.39→29.97 Å / Cor.coef. Fo:Fc: 0.952 / Cor.coef. Fo:Fc free: 0.918 / SU B: 8.064 / SU ML: 0.189 / Isotropic thermal model: ISOTROPIC / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.403 / ESU R Free: 0.251 / Stereochemistry target values: MAXIMUM LIKELIHOOD
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 36.634 Å2
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Refinement step | Cycle: LAST / Resolution: 2.39→29.97 Å
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Refine LS restraints |
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Refine LS restraints NCS | Dom-ID: 1 / Auth asym-ID: A / Ens-ID: 1 / Refine-ID: X-RAY DIFFRACTION
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LS refinement shell | Resolution: 2.395→2.456 Å / Total num. of bins used: 20
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