温度: 291 K / 手法: microbatch under oil / pH: 8 詳細: 2 micro-L of protein solution containing 10 mM Tris-HCl pH 7.5, 100 mM Sodium chloride, 5 mM DTT was mixed with equal volume of 100 mM Tris-HCl pH 8.0, 40% PEG 1000, and 100 mM Magnesium ...詳細: 2 micro-L of protein solution containing 10 mM Tris-HCl pH 7.5, 100 mM Sodium chloride, 5 mM DTT was mixed with equal volume of 100 mM Tris-HCl pH 8.0, 40% PEG 1000, and 100 mM Magnesium acetate, MICROBATCH UNDER OIL, temperature 291K
解像度: 2.1→2.18 Å / 冗長度: 6 % / Rmerge(I) obs: 0.329 / Mean I/σ(I) obs: 4 / Num. unique all: 2532 / Rsym value: 0.317 / % possible all: 99.5
-
解析
ソフトウェア
名称
バージョン
分類
CNS
1.1
精密化
ADSC
QUANTUM
データ収集
HKL-2000
データ削減
SCALEPACK
データスケーリング
SnB
位相決定
RESOLVE
位相決定
精密化
構造決定の手法: 単波長異常分散 / 解像度: 2.1→28.53 Å / Rfactor Rfree error: 0.006 / Data cutoff high absF: 755895.08 / Data cutoff low absF: 0 / Isotropic thermal model: OVERALL / 交差検証法: THROUGHOUT / σ(F): 2 / 立体化学のターゲット値: Engh & Huber 詳細: XtalView program has also been used in refinement. THE FRIEDEL PAIRS WERE USED FOR PHASING