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- PDB-2o0x: Mycobacterium tuberculosis epsp synthase in complex with intermediate -
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Open data
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Basic information
Entry | Database: PDB / ID: 2o0x | ||||||
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Title | Mycobacterium tuberculosis epsp synthase in complex with intermediate | ||||||
![]() | 3-phosphoshikimate 1-carboxyvinyltransferase | ||||||
![]() | TRANSFERASE / SHIKIMATE PATHWAY / EPSP SYNTHASE / M.TUBERCULOSIS / Structural Genomics / Mycobacterium Tuberculosis Structural Proteomics Project / XMTB | ||||||
Function / homology | ![]() 3-phosphoshikimate 1-carboxyvinyltransferase / 3-phosphoshikimate 1-carboxyvinyltransferase activity / Chorismate via Shikimate Pathway / chorismate biosynthetic process / aromatic amino acid family biosynthetic process / amino acid biosynthetic process / plasma membrane / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Kachalova, G.S. / Bartunik, H.D. / Mycobacterium Tuberculosis Structural Proteomics Project (XMTB) | ||||||
![]() | ![]() Title: Complexes of 3-PHOSPHOSHIKIMATE 1-CARBOXYVINYLTRANSFERASE Authors: Kachalova, G.S. / Burenkov, G.P. / Strizhov, N.I. / Brunning, M.G. / Bartunik, H.D. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 114.5 KB | Display | ![]() |
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PDB format | ![]() | 85 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 2o0dSC ![]() 2o0eC ![]() 2o0zC ![]() 2o15C S: Starting model for refinement C: citing same article ( |
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Similar structure data | |
Other databases |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
#1: Protein | Mass: 46473.656 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() References: UniProt: P22487, UniProt: P9WPY5*PLUS, 3-phosphoshikimate 1-carboxyvinyltransferase | ||||
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#2: Chemical | #3: Chemical | ChemComp-SKP / | #4: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.86 Å3/Da / Density % sol: 40.23 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 7.2 Details: 0.2M Ammonium sulfate ; 30%PEG 6000; 5mM S3P, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() |
Detector | Type: MAR CCD 165 mm / Detector: CCD / Date: Nov 25, 2004 / Details: Au-coated planar; toroidal mirrors |
Radiation | Monochromator: Si111 / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.05 Å / Relative weight: 1 |
Reflection | Resolution: 1.95→20 Å / Num. all: 28183 / Num. obs: 27055 / % possible obs: 95.9 % / Observed criterion σ(I): 2 / Redundancy: 5.5 % / Biso Wilson estimate: 24.1 Å2 / Rmerge(I) obs: 0.103 / Net I/σ(I): 13 |
Reflection shell | Resolution: 1.95→1.98 Å / Redundancy: 5 % / Rmerge(I) obs: 0.65 / Mean I/σ(I) obs: 2 / Num. unique all: 1104 / % possible all: 89 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 2O0D Resolution: 1.96→10 Å / Num. parameters: 15858 / Num. restraintsaints: 14301 / Isotropic thermal model: isotropic / σ(F): 0 / Stereochemistry target values: ENGH AND HUBER
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Displacement parameters | Biso mean: 23.9 Å2 | |||||||||||||||||||||||||||||||||
Refine analyze | Num. disordered residues: 63 / Occupancy sum hydrogen: 0 / Occupancy sum non hydrogen: 3698.8 | |||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.96→10 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.96→1.98 Å
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