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- PDB-2o15: Mycobacterium tuberculosis epsp synthase after partial products w... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2o15 | ||||||
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Title | Mycobacterium tuberculosis epsp synthase after partial products withdrawal | ||||||
![]() | 3-PHOSPHOSHIKIMATE 1-CARBOXYVINYLTRANSFERASE | ||||||
![]() | TRANSFERASE / SHIKIMATE PATHWAY / EPSP SYNTHASE / M.TUBERCULOSIS / Structural Genomics / Mycobacterium Tuberculosis Structural Proteomics Project / XMTB | ||||||
Function / homology | ![]() 3-phosphoshikimate 1-carboxyvinyltransferase / 3-phosphoshikimate 1-carboxyvinyltransferase activity / Chorismate via Shikimate Pathway / shikimate kinase activity / shikimate 3-dehydrogenase (NADP+) activity / 3-dehydroquinate dehydratase activity / chorismate biosynthetic process / aromatic amino acid family biosynthetic process / amino acid biosynthetic process / plasma membrane ...3-phosphoshikimate 1-carboxyvinyltransferase / 3-phosphoshikimate 1-carboxyvinyltransferase activity / Chorismate via Shikimate Pathway / shikimate kinase activity / shikimate 3-dehydrogenase (NADP+) activity / 3-dehydroquinate dehydratase activity / chorismate biosynthetic process / aromatic amino acid family biosynthetic process / amino acid biosynthetic process / plasma membrane / cytoplasm / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Kachalova, G.S. / Bartunik, H.D. / Mycobacterium Tuberculosis Structural Proteomics Project (XMTB) | ||||||
![]() | ![]() Title: Complexes of 3-PHOSPHOSHIKIMATE 1-CARBOXYVINYLTRANSFERASE Authors: Kachalova, G.S. / Burenkov, G.P. / Strizhov, N.I. / Brunning, M.G. / Bartunik, H.D. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 96 KB | Display | ![]() |
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PDB format | ![]() | 71.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 441.8 KB | Display | ![]() |
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Full document | ![]() | 462.1 KB | Display | |
Data in XML | ![]() | 21.4 KB | Display | |
Data in CIF | ![]() | 29.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 2o0dSC ![]() 2o0eC ![]() 2o0xC ![]() 2o0zC S: Starting model for refinement C: citing same article ( |
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Similar structure data | |
Other databases |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
#1: Protein | Mass: 46473.656 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() References: UniProt: P22487, UniProt: P9WPY5*PLUS, 3-phosphoshikimate 1-carboxyvinyltransferase | ||
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#2: Chemical | ChemComp-SO4 / | ||
#3: Chemical | #4: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.86 Å3/Da / Density meas: 34 Mg/m3 / Density % sol: 40.25 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 7.2 Details: 0.2M Ammonium sulfate ; 30%PEG 6000; 5mM S3P, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() |
Detector | Type: MAR CCD 165 mm / Detector: CCD / Date: Feb 20, 2005 / Details: Au-coated planar; toroidal mirrors |
Radiation | Monochromator: Si111 / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.05 Å / Relative weight: 1 |
Reflection | Resolution: 1.95→20 Å / Num. all: 29110 / Num. obs: 28417 / % possible obs: 98.6 % / Observed criterion σ(I): 2 / Redundancy: 3 % / Biso Wilson estimate: 36.2 Å2 / Rmerge(I) obs: 0.057 / Net I/σ(I): 21 |
Reflection shell | Resolution: 1.95→1.98 Å / Redundancy: 3 % / Rmerge(I) obs: 0.419 / Mean I/σ(I) obs: 2.65 / Num. unique all: 1392 / % possible all: 96.2 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 2O0D Resolution: 1.95→10 Å / Num. parameters: 13275 / Num. restraintsaints: 13237 / Isotropic thermal model: isotropic / σ(F): 0 / Stereochemistry target values: ENGH AND HUBER
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Displacement parameters | Biso mean: 36.15 Å2 | |||||||||||||||||||||||||||||||||
Refine analyze | Num. disordered residues: 9 / Occupancy sum hydrogen: 0 / Occupancy sum non hydrogen: 3152.7 | |||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.95→10 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.95→1.98 Å
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