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Yorodumi- PDB-2npr: Structural Studies on Plasmodium vivax Merozoite Surface Protein-1 -
+Open data
-Basic information
Entry | Database: PDB / ID: 2npr | ||||||
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Title | Structural Studies on Plasmodium vivax Merozoite Surface Protein-1 | ||||||
Components | Merozoite surface protein 1 | ||||||
Keywords | MEMBRANE PROTEIN / EGF-LIKE DOMAIN | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Plasmodium vivax (malaria parasite P. vivax) | ||||||
Method | SOLUTION NMR / SIMMULATED ANNEALING WITH TAD | ||||||
Authors | Babon, J.J. / Morgan, W.D. / Kelly, G. / Eccleston, J.F. / Feeney, J. / Holder, A.A. | ||||||
Citation | Journal: Mol.Biochem.Parasitol. / Year: 2007 Title: Structural studies on Plasmodium vivax merozoite surface protein-1 Authors: Babon, J.J. / Morgan, W.D. / Kelly, G. / Eccleston, J.F. / Feeney, J. / Holder, A.A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2npr.cif.gz | 507.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2npr.ent.gz | 422.7 KB | Display | PDB format |
PDBx/mmJSON format | 2npr.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2npr_validation.pdf.gz | 345.3 KB | Display | wwPDB validaton report |
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Full document | 2npr_full_validation.pdf.gz | 574.8 KB | Display | |
Data in XML | 2npr_validation.xml.gz | 62.6 KB | Display | |
Data in CIF | 2npr_validation.cif.gz | 82.2 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/np/2npr ftp://data.pdbj.org/pub/pdb/validation_reports/np/2npr | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 9823.011 Da / Num. of mol.: 1 / Fragment: C-TERMINAL DOMAIN, residues 1-90 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Plasmodium vivax (strain Belem) (eukaryote) Species: Plasmodium vivax / Strain: BELEM / Gene: Msp1 / Plasmid: pET-30Xa/LIC / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21 (DE3) / References: UniProt: Q2I7F0, UniProt: Q8I1L1*PLUS |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||
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NMR experiment |
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NMR details | Text: The structure was determined using triple-resonance NMR spectroscopy. |
-Sample preparation
Details | Contents: U-13C,15N MSP1; 20mM K-PHOSPHATE BUFFER(pH 6.5); 90% H2O, 10% D2O Solvent system: 90% H2O/10% D2O |
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Sample conditions | Ionic strength: 20mM / pH: 6.5 / Pressure: 1 atm / Temperature: 303 K |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: SIMMULATED ANNEALING WITH TAD / Software ordinal: 1 / Details: XPLOR | |||||||||
NMR representative | Selection criteria: lowest energy | |||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 20 |