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Yorodumi- PDB-2nnz: Solution structure of the hypothetical protein AF2241 from Archae... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2nnz | ||||||
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Title | Solution structure of the hypothetical protein AF2241 from Archaeoglobus fulgidus | ||||||
Components | Hypothetical protein | ||||||
Keywords | STRUCTURAL GENOMICS / UNKNOWN FUNCTION / beta-barrel / Ontario Centre for Structural Proteomics / OCSP | ||||||
Function / homology | TM1367-like / Cyclophilin TM1367-like domain / Cyclophilin-like / Cyclophilin - #20 / Cyclophilin / Cyclophilin-like domain superfamily / Beta Barrel / Mainly Beta / Cyclophil_like domain-containing protein Function and homology information | ||||||
Biological species | Archaeoglobus fulgidus (archaea) | ||||||
Method | SOLUTION NMR / simulated annealing, torsion angle dynamics | ||||||
Authors | Ai, X. / Semesib, A. / Yee, A. / Arrowsmith, C.H. / Li, S.S.C. / Choy, W.Y. / Ontario Centre for Structural Proteomics (OCSP) | ||||||
Citation | Journal: J.Biomol.Nmr / Year: 2007 Title: Hypothetical protein AF2241 from Archaeoglobus fulgidus adopts a cyclophilin-like fold. Authors: Ai, X. / Li, L. / Semesi, A. / Yee, A. / Arrowsmith, C.H. / Li, S.S. / Choy, W.Y. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2nnz.cif.gz | 791.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2nnz.ent.gz | 657 KB | Display | PDB format |
PDBx/mmJSON format | 2nnz.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2nnz_validation.pdf.gz | 541.1 KB | Display | wwPDB validaton report |
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Full document | 2nnz_full_validation.pdf.gz | 773.1 KB | Display | |
Data in XML | 2nnz_validation.xml.gz | 76.5 KB | Display | |
Data in CIF | 2nnz_validation.cif.gz | 95.2 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/nn/2nnz ftp://data.pdbj.org/pub/pdb/validation_reports/nn/2nnz | HTTPS FTP |
-Related structure data
Similar structure data | |
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Other databases |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 17135.246 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Archaeoglobus fulgidus (archaea) / Strain: DSM 4304 / Gene: AF2241 / Plasmid: pMgk / Production host: Escherichia coli (E. coli) / Strain (production host): BL21-(Gold DE3) / References: UniProt: O28042 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
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NMR experiment |
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NMR details | Text: The structure was determined using standard 3D heteronuclear techniques |
-Sample preparation
Details | Contents: 0.3mM AF2241 U-15N,13C, 10mM MOPS buffer, 450mM NaCl, 10uM Zn2+, 10mM DTT, 1mM benzamidine, 1x inhibitor mixture, 0.01% NaN3, 90% H2O, 10% D2O Solvent system: 90% H2O/10% D2O |
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Sample conditions | Ionic strength: 450 mM NaCl / pH: 6.5 / Pressure: 1 atm / Temperature: 298 K |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
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Radiation wavelength | Relative weight: 1 |
NMR spectrometer | Type: Varian INOVA / Manufacturer: Varian / Model: INOVA / Field strength: 600 MHz |
-Processing
NMR software |
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Refinement | Method: simulated annealing, torsion angle dynamics / Software ordinal: 1 Details: The structures are based on a total of 1463 restraints, 1313 are NOE-derived distance constraints and 150 are dihedral angle restraints | ||||||||||||
NMR representative | Selection criteria: closest to the average | ||||||||||||
NMR ensemble | Conformer selection criteria: all calculated structures submitted Conformers calculated total number: 20 / Conformers submitted total number: 20 |