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- PDB-2nmb: DNUMB PTB DOMAIN COMPLEXED WITH A PHOSPHOTYROSINE PEPTIDE, NMR, E... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2nmb | ||||||
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Title | DNUMB PTB DOMAIN COMPLEXED WITH A PHOSPHOTYROSINE PEPTIDE, NMR, ENSEMBLE OF STRUCTURES. | ||||||
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![]() | CELL CYCLE/GENE REGULATION / COMPLEX / SIGNAL TRANSDUCTION / PHOSPHOTYROSINE BINDING DOMAIN (PTB) / ASYMETR IC CELL DIVISION / CELL CYCLE-GENE REGULATION COMPLEX | ||||||
Function / homology | ![]() pericardial nephrocyte differentiation / enteroendocrine cell differentiation / Malpighian tubule tip cell differentiation / regulation of nervous system development / sensory organ precursor cell fate determination / sensory organ precursor cell division / neuroblast development / muscle cell fate specification / regulation of asymmetric cell division / regulation of neuroblast proliferation ...pericardial nephrocyte differentiation / enteroendocrine cell differentiation / Malpighian tubule tip cell differentiation / regulation of nervous system development / sensory organ precursor cell fate determination / sensory organ precursor cell division / neuroblast development / muscle cell fate specification / regulation of asymmetric cell division / regulation of neuroblast proliferation / negative regulation of receptor recycling / glial cell migration / asymmetric neuroblast division / basal part of cell / embryonic heart tube development / Notch binding / centrosome localization / positive regulation of neurogenesis / negative regulation of neuroblast proliferation / negative regulation of Notch signaling pathway / positive regulation of endocytosis / regulation of neurogenesis / neuroblast proliferation / protein localization / cell cortex / negative regulation of gene expression / ATP binding / nucleus / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | SOLUTION NMR / SIMULATED ANNEALING WITH AMBIGUOUS RESTRAINTS | ||||||
![]() | Li, S.-C. / Zwahlen, C. / Vincent, S.J.F. / McGlade, C.J. / Pawson, T. / Forman-Kay, J.D. | ||||||
![]() | ![]() Title: Structure of a Numb PTB domain-peptide complex suggests a basis for diverse binding specificity. Authors: Li, S.C. / Zwahlen, C. / Vincent, S.J. / McGlade, C.J. / Kay, L.E. / Pawson, T. / Forman-Kay, J.D. #1: ![]() Title: High-Affinity Binding of the Drosophila Numb Phosphotyrosine-Binding Domain to Pep Tides Containing a Gly-Pro-(P)Tyr Motif Authors: Li, S.-C. / Songyang, Z. / Vincent, S.J.F. / Zwahlen, C. / Wiley, S. / Cantley, L. / Kay, L.E. / Forman-Kay, J.D. / Pawson, T. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 666.2 KB | Display | ![]() |
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PDB format | ![]() | 552.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 366.7 KB | Display | ![]() |
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Full document | ![]() | 599 KB | Display | |
Data in XML | ![]() | 56.9 KB | Display | |
Data in CIF | ![]() | 71.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: Protein | Mass: 17914.402 Da / Num. of mol.: 1 / Fragment: PTB DOMAIN Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#2: Protein/peptide | Mass: 875.902 Da / Num. of mol.: 1 / Source method: obtained synthetically / Details: CHEMICALLY SYNTHESIZED |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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NMR details | Text: NOE MIXING TIME 50MS AND 90MS FOR N-NOESY-HSQC, C-HSQC-NOESY, CN-NOESY NOE MIXING TIME 150MS FOR HALF-FILTER-NOESY,FILTER-NOESY |
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Sample preparation
Sample conditions | pH: 6.0 / Pressure: 1 atm / Temperature: 303 K |
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Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer |
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Processing
NMR software |
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Refinement | Method: SIMULATED ANNEALING WITH AMBIGUOUS RESTRAINTS / Software ordinal: 1 Details: MODIFICATION OF X-PLOR TO INCORPORATE ARIA PROTOCOL FOR AMBIGUOUS NOE ASSIGNMENT. | ||||||||||||
NMR ensemble | Conformer selection criteria: NO NOE VIOLATION > 0.3 A / Conformers calculated total number: 200 / Conformers submitted total number: 14 |