手法: 溶液NMR 詳細: This variant of Penicillium Antifungal Protein (PAF) is produced by a aspartic acid to serine mutation of residue 19. By this single residue exchange the antifungal function of the protein ...詳細: This variant of Penicillium Antifungal Protein (PAF) is produced by a aspartic acid to serine mutation of residue 19. By this single residue exchange the antifungal function of the protein has been dramatically modified.
NMR実験
Conditions-ID
Experiment-ID
Solution-ID
タイプ
1
1
1
2D 1H-15N HSQC
1
2
1
2D 1H-13C HSQC
1
3
1
2D 1H-1H TOCSY
1
4
1
2D 1H-1H NOESY
1
5
1
3D HNHA
1
6
1
3D 1H-15N NOESY
1
7
1
3D 1H-15N TOCSY
1
8
1
2D 1H-15N HSQC
1
9
1
2D 1H-1H NOESY
-
試料調製
詳細
内容: 1.7 mM [U-100% 15N] PAFD19S, 5 v/v [U-2H] D2O, 40 mM sodium chloride, 10 mM sodium phosphate, 0.04 % potassium phosphate, 95% H2O/5% D2O 溶媒系: 95% H2O/5% D2O