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Yorodumi- PDB-2n5g: NMR structure of KorA, a plasmid-encoded, global transcription re... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2n5g | ||||||
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Title | NMR structure of KorA, a plasmid-encoded, global transcription regulator KorA | ||||||
Components | TrfB transcriptional repressor protein | ||||||
Keywords | TRANSCRIPTION | ||||||
Function / homology | TrfB transcriptional repressor protein / TrfB plasmid transcriptional repressor / DNA binding / TrfB transcriptional repressor protein Function and homology information | ||||||
Biological species | Escherichia coli (E. coli) | ||||||
Method | SOLUTION NMR / torsion angle dynamics | ||||||
Model details | lowest energy, model167 | ||||||
Authors | Rajasekar, K.V. / Lovering, A.L. / Dancea, F.V. / Scott, D.J. / Harris, S. / Bingle, L.E. / Roessle, M. / Thomas, C.M. / Hyde, E.I. / White, S.A. | ||||||
Citation | Journal: Nucleic Acids Res. / Year: 2016 Title: Flexibility of KorA, a plasmid-encoded, global transcription regulator, in the presence and the absence of its operator. Authors: Rajasekar, K.V. / Lovering, A.L. / Dancea, F. / Scott, D.J. / Harris, S.A. / Bingle, L.E. / Roessle, M. / Thomas, C.M. / Hyde, E.I. / White, S.A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2n5g.cif.gz | 1.2 MB | Display | PDBx/mmCIF format |
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PDB format | pdb2n5g.ent.gz | 1 MB | Display | PDB format |
PDBx/mmJSON format | 2n5g.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2n5g_validation.pdf.gz | 481.9 KB | Display | wwPDB validaton report |
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Full document | 2n5g_full_validation.pdf.gz | 1.2 MB | Display | |
Data in XML | 2n5g_validation.xml.gz | 176.7 KB | Display | |
Data in CIF | 2n5g_validation.cif.gz | 149.9 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/n5/2n5g ftp://data.pdbj.org/pub/pdb/validation_reports/n5/2n5g | HTTPS FTP |
-Related structure data
Related structure data | 5cktC 5clvC 5cm3C C: citing same article (ref.) |
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Similar structure data | |
Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 11323.908 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Gene: trfB, korA / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: P03052 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR / Details: KorA Repressor Protein | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 1 mM [U-13C; U-15N] KorA, 25 mM Phosphate Buffer, 0.1 mM EDTA, 95% H2O/5% D2O Solvent system: 95% H2O/5% D2O | ||||||||||||||||
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Sample |
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Sample conditions | pH: 6.2 / Temperature: 303 K |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: torsion angle dynamics / Software ordinal: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 200 / Conformers submitted total number: 20 |