+Open data
-Basic information
Entry | Database: PDB / ID: 2mvy | ||||||
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Title | Structure and Stability of RNAs Containing N6-Methyl-adenosine | ||||||
Components | RNA | ||||||
Keywords | RNA / RNA duplex | ||||||
Function / homology | RNA Function and homology information | ||||||
Method | SOLUTION NMR / simulated annealing, molecular dynamics | ||||||
Model details | minimized average structure, model 1 | ||||||
Model type details | minimized average | ||||||
Authors | Lynch, S.R. / Kool, E.T. | ||||||
Citation | Journal: J.Am.Chem.Soc. / Year: 2015 Title: Structure and thermodynamics of N6-methyladenosine in RNA: a spring-loaded base modification. Authors: Roost, C. / Lynch, S.R. / Batista, P.J. / Qu, K. / Chang, H.Y. / Kool, E.T. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2mvy.cif.gz | 21.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2mvy.ent.gz | 14 KB | Display | PDB format |
PDBx/mmJSON format | 2mvy.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/mv/2mvy ftp://data.pdbj.org/pub/pdb/validation_reports/mv/2mvy | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: RNA chain | Mass: 3176.948 Da / Num. of mol.: 2 / Source method: obtained synthetically |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample |
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Sample conditions | Ionic strength: 100 / pH: 6.4 / Pressure: 1 atm / Temperature: 298 K |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: simulated annealing, molecular dynamics / Software ordinal: 1 | ||||||||||||||||||||||||||||
NMR constraints | NA alpha-angle constraints total count: 0 / NA beta-angle constraints total count: 18 / NA chi-angle constraints total count: 20 / NA epsilon-angle constraints total count: 18 / NA gamma-angle constraints total count: 20 / NA other-angle constraints total count: 20 / NA sugar pucker constraints total count: 80 / NOE constraints total: 342 / NOE intraresidue total count: 160 / NOE long range total count: 38 / NOE medium range total count: 0 / NOE sequential total count: 144 / Hydrogen bond constraints total count: 40 | ||||||||||||||||||||||||||||
NMR representative | Selection criteria: minimized average structure | ||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: target function / Conformers calculated total number: 30 / Conformers submitted total number: 1 |