+Open data
-Basic information
Entry | Database: PDB / ID: 2mrm | ||||||
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Title | Solution structure of the rhodanese domain of YgaP from E. coli | ||||||
Components | Membrane protein | ||||||
Keywords | MEMBRANE PROTEIN / rhodanese domain / YgaP / E. coli / Integral Membrane Protein | ||||||
Function / homology | Rhodanese-like domain / Oxidized Rhodanese; domain 1 / 3-Layer(aba) Sandwich / Alpha Beta / : Function and homology information | ||||||
Biological species | Escherichia coli (E. coli) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Model details | lowest energy, model1 | ||||||
Authors | Wang, W. / Zhou, P. / Tian, C. / Wu, F. | ||||||
Citation | Journal: Biochem.Biophys.Res.Commun. / Year: 2014 Title: Fast conformational exchange between the sulfur-free and persulfide-bound rhodanese domain of E. coli YgaP Authors: Wang, W. / Zhou, P. / He, Y. / Yu, L. / Xiong, Y. / Tian, C. / Wu, F. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2mrm.cif.gz | 329.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2mrm.ent.gz | 269.8 KB | Display | PDB format |
PDBx/mmJSON format | 2mrm.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2mrm_validation.pdf.gz | 405.6 KB | Display | wwPDB validaton report |
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Full document | 2mrm_full_validation.pdf.gz | 507.8 KB | Display | |
Data in XML | 2mrm_validation.xml.gz | 29.4 KB | Display | |
Data in CIF | 2mrm_validation.cif.gz | 40.4 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/mr/2mrm ftp://data.pdbj.org/pub/pdb/validation_reports/mr/2mrm | HTTPS FTP |
-Related structure data
Similar structure data | |
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Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 12620.390 Da / Num. of mol.: 1 / Fragment: UNP residues 1-107 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Gene: BU34_15760, CF57_07085, CF61_07785 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21-Gold(DE3) / References: UniProt: W8T678 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample |
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Sample conditions | Ionic strength: 0.05 / pH: 7 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
NMR spectrometer | Type: Varian INOVA / Manufacturer: Varian / Model: INOVA / Field strength: 700 MHz |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | |||||||||||||||||||||
NMR constraints | NOE constraints total: 1875 / NOE intraresidue total count: 345 / NOE long range total count: 519 / NOE medium range total count: 459 / NOE sequential total count: 552 / Protein phi angle constraints total count: 63 / Protein psi angle constraints total count: 63 | |||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | |||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 10 / Representative conformer: 1 |