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Yorodumi- PDB-2mog: Solution structure of the terminal Ig-like domain from Leptospira... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2mog | ||||||
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Title | Solution structure of the terminal Ig-like domain from Leptospira interrogans LigB | ||||||
Components | Bacterial Ig-like domain, group 2 | ||||||
Keywords | CELL ADHESION / Immunoglobulin-like fold / surface protein | ||||||
Function / homology | Immunoglobulin-like - #1080 / Immunoglobulin-like / Sandwich / Mainly Beta / : Function and homology information | ||||||
Biological species | Leptospira interrogans (bacteria) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Model details | lowest energy, model1 | ||||||
Authors | Ptak, C.P. / Hsieh, C. / Lin, Y. / Maltsev, A.S. / Raman, R. / Sharma, Y. / Oswald, R.E. / Chang, Y. | ||||||
Citation | Journal: Biochemistry / Year: 2014 Title: NMR Solution Structure of the Terminal Immunoglobulin-like Domain from the Leptospira Host-Interacting Outer Membrane Protein, LigB. Authors: Ptak, C.P. / Hsieh, C.L. / Lin, Y.P. / Maltsev, A.S. / Raman, R. / Sharma, Y. / Oswald, R.E. / Chang, Y.F. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2mog.cif.gz | 521.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2mog.ent.gz | 438.6 KB | Display | PDB format |
PDBx/mmJSON format | 2mog.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2mog_validation.pdf.gz | 539.2 KB | Display | wwPDB validaton report |
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Full document | 2mog_full_validation.pdf.gz | 784.5 KB | Display | |
Data in XML | 2mog_validation.xml.gz | 67 KB | Display | |
Data in CIF | 2mog_validation.cif.gz | 71.1 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/mo/2mog ftp://data.pdbj.org/pub/pdb/validation_reports/mo/2mog | HTTPS FTP |
-Related structure data
Similar structure data | |
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Other databases |
-Links
-Assembly
Deposited unit |
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NMR ensembles |
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-Components
#1: Protein | Mass: 9641.489 Da / Num. of mol.: 1 / Fragment: Ig-like domain 12 (UNP residues 1029-1123) Source method: isolated from a genetically manipulated source Source: (gene. exp.) Leptospira interrogans (bacteria) / Gene: LEP1GSC112_5106 / Plasmid: pET28-His-Sumo / Production host: Escherichia coli (E. coli) / References: UniProt: M6R7K6 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample |
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Sample conditions | Ionic strength: 0.15 / pH: 7 / Pressure: ambient / Temperature: 286 K |
-NMR measurement
NMR spectrometer | Type: Varian INOVA / Manufacturer: Varian / Model: INOVA / Field strength: 500 MHz |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | |||||||||
NMR representative | Selection criteria: lowest energy | |||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 20 |