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Yorodumi- PDB-2mjf: Solution structure of the complex between the yeast Rsa1 and Hit1... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2mjf | ||||||
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Title | Solution structure of the complex between the yeast Rsa1 and Hit1 proteins | ||||||
Components |
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Keywords | PROTEIN BINDING | ||||||
Function / homology | Function and homology information snoRNA localization / pre-snoRNP complex / box C/D snoRNP assembly / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / ribosomal large subunit assembly / nucleoplasm / nucleus / metal ion binding / cytoplasm Similarity search - Function | ||||||
Biological species | Saccharomyces cerevisiae (brewer's yeast) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Model details | lowest energy, model 1 | ||||||
Authors | Quinternet, M. / Roth, B. / Back, R. / Jacquemin, C. / Manival, X. | ||||||
Citation | Journal: Nucleic Acids Res. / Year: 2014 Title: Protein Hit1, a novel box C/D snoRNP assembly factor, controls cellular concentration of the scaffolding protein Rsa1 by direct interaction. Authors: Rothe, B. / Saliou, J.M. / Quinternet, M. / Back, R. / Tiotiu, D. / Jacquemin, C. / Loegler, C. / Schlotter, F. / Pena, V. / Eckert, K. / Morera, S. / Dorsselaer, A.V. / Branlant, C. / ...Authors: Rothe, B. / Saliou, J.M. / Quinternet, M. / Back, R. / Tiotiu, D. / Jacquemin, C. / Loegler, C. / Schlotter, F. / Pena, V. / Eckert, K. / Morera, S. / Dorsselaer, A.V. / Branlant, C. / Massenet, S. / Sanglier-Cianferani, S. / Manival, X. / Charpentier, B. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2mjf.cif.gz | 843.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2mjf.ent.gz | 707.2 KB | Display | PDB format |
PDBx/mmJSON format | 2mjf.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2mjf_validation.pdf.gz | 420.5 KB | Display | wwPDB validaton report |
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Full document | 2mjf_full_validation.pdf.gz | 663.4 KB | Display | |
Data in XML | 2mjf_validation.xml.gz | 41.6 KB | Display | |
Data in CIF | 2mjf_validation.cif.gz | 70.1 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/mj/2mjf ftp://data.pdbj.org/pub/pdb/validation_reports/mj/2mjf | HTTPS FTP |
-Related structure data
Similar structure data | |
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Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein/peptide | Mass: 4543.007 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Strain: ATCC 204508 / S288c / Gene: RSA1, YPL193W / Production host: Escherichia coli (E. coli) / References: UniProt: Q08932 |
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#2: Protein | Mass: 10920.488 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Strain: ATCC 204508 / S288c / Gene: HIT1, YJR055W, J1705 / Production host: Escherichia coli (E. coli) / References: UniProt: P46973 |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 1 mM [U-100% 13C; U-100% 15N] rsa, 1 mM [U-100% 13C; U-100% 15N] hit, 150 mM sodium chloride, 90 % H2O, 10 % [U-100% 2H] D2O, 90% H2O/10% D2O Solvent system: 90% H2O/10% D2O | ||||||||||||||||||||||||
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Sample |
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Sample conditions | Ionic strength: 150 / pH: 6.4 / Pressure: ambient / Temperature: 293 K |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 200 / Conformers submitted total number: 20 |