1 mM [U-100% 13C; U-100% 15N] Sp140 PHD finger trans conformer, 50 uM ZINC ION, 0.3 mM DSS, 5 mM DTT, 20 mM sodium phosphate pH 6.3, 150 mM NaCl, 90% H2O/10% D2O
90% H2O/10% D2O
2
1 mM [U-100% 13C; U-100% 15N] Sp140 PHD finger trans conformer, 50 uM ZINC ION, 0.3 mM DSS, 5 mM DTT, 20 mM sodium phosphate pH 6.3, 150 mM NaCl, 100% D2O
100% D2O
3
0.8 mM [U-100% 15N] Sp140 PHD finger trans conformer, 50 uM ZINC ION, 0.3 mM DSS, 5 mM DTT, 20 mM sodium phosphate pH 6.3, 150 mM NaCl, 90% H2O/10% D2O
NOE constraints total: 884 / NOE intraresidue total count: 435 / NOE long range total count: 76 / NOE medium range total count: 131 / NOE sequential total count: 233 / Protein chi angle constraints total count: 0 / Protein other angle constraints total count: 0 / Protein phi angle constraints total count: 23 / Protein psi angle constraints total count: 23
代表構造
選択基準: lowest energy
NMRアンサンブル
コンフォーマー選択の基準: structures with the lowest energy 計算したコンフォーマーの数: 100 / 登録したコンフォーマーの数: 20