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- PDB-6cfn: Crystal Structure of the DNA-free Glucocorticoid Receptor DNA Bin... -

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Basic information

Entry
Database: PDB / ID: 6cfn
TitleCrystal Structure of the DNA-free Glucocorticoid Receptor DNA Binding Domain
ComponentsGlucocorticoid receptor
KeywordsDNA BINDING PROTEIN / DNA binding domain / nuclear receptor / glucocorticoid receptor
Function / homology
Function and homology information


Regulation of NPAS4 gene transcription / regulation of glucocorticoid biosynthetic process / nuclear glucocorticoid receptor activity / steroid hormone binding / PTK6 Expression / neuroinflammatory response / glucocorticoid metabolic process / microglia differentiation / mammary gland duct morphogenesis / maternal behavior ...Regulation of NPAS4 gene transcription / regulation of glucocorticoid biosynthetic process / nuclear glucocorticoid receptor activity / steroid hormone binding / PTK6 Expression / neuroinflammatory response / glucocorticoid metabolic process / microglia differentiation / mammary gland duct morphogenesis / maternal behavior / astrocyte differentiation / cellular response to glucocorticoid stimulus / motor behavior / regulation of gluconeogenesis / adrenal gland development / cellular response to steroid hormone stimulus / estrogen response element binding / FOXO-mediated transcription of oxidative stress, metabolic and neuronal genes / nuclear receptor-mediated steroid hormone signaling pathway / core promoter sequence-specific DNA binding / cellular response to transforming growth factor beta stimulus / HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand / TBP-class protein binding / steroid binding / cellular response to dexamethasone stimulus / synaptic transmission, glutamatergic / chromosome segregation / RNA polymerase II transcription regulatory region sequence-specific DNA binding / Hsp90 protein binding / SUMOylation of intracellular receptors / DNA-binding transcription repressor activity, RNA polymerase II-specific / positive regulation of miRNA transcription / Nuclear Receptor transcription pathway / spindle / Regulation of RUNX2 expression and activity / nuclear receptor activity / sequence-specific double-stranded DNA binding / positive regulation of neuron apoptotic process / Circadian Clock / chromatin organization / gene expression / DNA-binding transcription activator activity, RNA polymerase II-specific / Potential therapeutics for SARS / DNA-binding transcription factor activity, RNA polymerase II-specific / nuclear speck / mitochondrial matrix / RNA polymerase II cis-regulatory region sequence-specific DNA binding / DNA-binding transcription factor activity / cell division / negative regulation of DNA-templated transcription / centrosome / apoptotic process / synapse / regulation of DNA-templated transcription / chromatin / regulation of transcription by RNA polymerase II / protein kinase binding / negative regulation of transcription by RNA polymerase II / signal transduction / positive regulation of transcription by RNA polymerase II / protein-containing complex / RNA binding / zinc ion binding / nucleoplasm / identical protein binding / membrane / nucleus / cytoplasm / cytosol
Similarity search - Function
Glucocorticoid receptor / Glucocorticoid receptor / Erythroid Transcription Factor GATA-1, subunit A / Erythroid Transcription Factor GATA-1; Chain A / Nuclear hormone receptor / Nuclear hormones receptors DNA-binding region signature. / Zinc finger, nuclear hormone receptor-type / Zinc finger, C4 type (two domains) / Nuclear hormone receptors DNA-binding domain profile. / c4 zinc finger in nuclear hormone receptors ...Glucocorticoid receptor / Glucocorticoid receptor / Erythroid Transcription Factor GATA-1, subunit A / Erythroid Transcription Factor GATA-1; Chain A / Nuclear hormone receptor / Nuclear hormones receptors DNA-binding region signature. / Zinc finger, nuclear hormone receptor-type / Zinc finger, C4 type (two domains) / Nuclear hormone receptors DNA-binding domain profile. / c4 zinc finger in nuclear hormone receptors / Nuclear hormone receptor, ligand-binding domain / Nuclear hormone receptor-like domain superfamily / Ligand-binding domain of nuclear hormone receptor / Nuclear receptor (NR) ligand-binding (LBD) domain profile. / Ligand binding domain of hormone receptors / Zinc finger, NHR/GATA-type / 2-Layer Sandwich / Alpha Beta
Similarity search - Domain/homology
Glucocorticoid receptor
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.5 Å
AuthorsFrank, F. / Okafor, C.D. / Ortlund, E.A.
Funding support United States, 1items
OrganizationGrant numberCountry
W. M. Keck Foundation United States
CitationJournal: Sci Rep / Year: 2018
Title: The first crystal structure of a DNA-free nuclear receptor DNA binding domain sheds light on DNA-driven allostery in the glucocorticoid receptor.
Authors: Frank, F. / Okafor, C.D. / Ortlund, E.A.
History
DepositionFeb 15, 2018Deposition site: RCSB / Processing site: RCSB
Revision 1.0Sep 26, 2018Provider: repository / Type: Initial release
Revision 1.1Oct 4, 2023Group: Data collection / Database references / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Glucocorticoid receptor
B: Glucocorticoid receptor
C: Glucocorticoid receptor
D: Glucocorticoid receptor
E: Glucocorticoid receptor
F: Glucocorticoid receptor
G: Glucocorticoid receptor
H: Glucocorticoid receptor
hetero molecules


Theoretical massNumber of molelcules
Total (without water)81,09324
Polymers80,0478
Non-polymers1,04716
Water46826
1
A: Glucocorticoid receptor
hetero molecules


Theoretical massNumber of molelcules
Total (without water)10,1373
Polymers10,0061
Non-polymers1312
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
B: Glucocorticoid receptor
hetero molecules


Theoretical massNumber of molelcules
Total (without water)10,1373
Polymers10,0061
Non-polymers1312
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
3
C: Glucocorticoid receptor
hetero molecules


Theoretical massNumber of molelcules
Total (without water)10,1373
Polymers10,0061
Non-polymers1312
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
4
D: Glucocorticoid receptor
hetero molecules


Theoretical massNumber of molelcules
Total (without water)10,1373
Polymers10,0061
Non-polymers1312
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
5
E: Glucocorticoid receptor
hetero molecules


Theoretical massNumber of molelcules
Total (without water)10,1373
Polymers10,0061
Non-polymers1312
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
6
F: Glucocorticoid receptor
hetero molecules


Theoretical massNumber of molelcules
Total (without water)10,1373
Polymers10,0061
Non-polymers1312
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
7
G: Glucocorticoid receptor
hetero molecules


Theoretical massNumber of molelcules
Total (without water)10,1373
Polymers10,0061
Non-polymers1312
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
8
H: Glucocorticoid receptor
hetero molecules


Theoretical massNumber of molelcules
Total (without water)10,1373
Polymers10,0061
Non-polymers1312
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)65.803, 65.432, 72.659
Angle α, β, γ (deg.)71.27, 84.96, 68.44
Int Tables number1
Space group name H-MP1

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Components

#1: Protein
Glucocorticoid receptor / GR / Nuclear receptor subfamily 3 group C member 1


Mass: 10005.842 Da / Num. of mol.: 8 / Mutation: UNP Residues 418-506
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: NR3C1, GRL / Plasmid: pSmt3 / Details (production host): His6-SUMO-tag / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: P04150
#2: Chemical
ChemComp-ZN / ZINC ION


Mass: 65.409 Da / Num. of mol.: 16 / Source method: obtained synthetically / Formula: Zn
#3: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 26 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.2 Å3/Da / Density % sol: 61 %
Crystal growTemperature: 291 K / Method: vapor diffusion, hanging drop / pH: 8.5
Details: 25% PEG3350, 50 mM Tris pH8.5, and 200 mM di-sodium tartrate

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 22-ID / Wavelength: 1 Å
DetectorType: MARMOSAIC 300 mm CCD / Detector: CCD / Date: Mar 3, 2017
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1 Å / Relative weight: 1
ReflectionResolution: 2.5→36 Å / Num. obs: 33141 / % possible obs: 89.5 % / Redundancy: 2.4 % / Rpim(I) all: 0.1 / Rrim(I) all: 0.18 / Χ2: 53.6 / Net I/σ(I): 14.5
Reflection shellResolution: 2.5→2.59 Å / Redundancy: 1.4 % / Mean I/σ(I) obs: 1.3 / Num. unique obs: 2095 / CC1/2: 0.56 / Rpim(I) all: 0.46 / Rrim(I) all: 0.65 / % possible all: 56.9

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Processing

Software
NameVersionClassification
PHENIX(1.11.1_2575: ???)refinement
HKL-2000data reduction
HKL-2000data scaling
PHENIXphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 4HN5

4hn5


Resolution: 2.5→36.042 Å / SU ML: 0.34 / Cross valid method: THROUGHOUT / σ(F): 1.97 / Phase error: 31.36 / Stereochemistry target values: ML
RfactorNum. reflection% reflectionSelection details
Rfree0.2483 1654 5.01 %Random election
Rwork0.1946 ---
obs0.1973 33027 89.5 %-
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Refinement stepCycle: LAST / Resolution: 2.5→36.042 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms4601 0 16 26 4643
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.0094663
X-RAY DIFFRACTIONf_angle_d1.1596207
X-RAY DIFFRACTIONf_dihedral_angle_d9.8282895
X-RAY DIFFRACTIONf_chiral_restr0.061663
X-RAY DIFFRACTIONf_plane_restr0.005795
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.5001-2.57370.3771860.30161613X-RAY DIFFRACTION56
2.5737-2.65670.30491070.27692032X-RAY DIFFRACTION70
2.6567-2.75160.38231260.27042389X-RAY DIFFRACTION81
2.7516-2.86180.29721390.26362643X-RAY DIFFRACTION90
2.8618-2.99190.32851470.26342774X-RAY DIFFRACTION95
2.9919-3.14960.30031500.22932843X-RAY DIFFRACTION97
3.1496-3.34680.27081490.22282830X-RAY DIFFRACTION97
3.3468-3.6050.27421520.20852866X-RAY DIFFRACTION98
3.605-3.96740.2231500.17452862X-RAY DIFFRACTION98
3.9674-4.54050.18241520.14232876X-RAY DIFFRACTION98
4.5405-5.71680.19621500.16182858X-RAY DIFFRACTION98
5.7168-36.04540.25511460.18612787X-RAY DIFFRACTION95
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
12.5993-3.77211.86346.5898-1.65262.308-0.05060.88311.6007-0.40740.0159-0.1943-0.9490.11460.26260.4397-0.05510.04950.75310.13420.61573.0826-9.1037-20.9016
25.91982.08371.03188.9884-2.38626.0514-0.5107-0.7491-0.7197-0.3185-0.0365-1.51650.11241.43990.42590.40310.06670.16230.6770.1070.61514.6878-16.4782-12.4939
32.00611.5996-0.56837.2452-1.58655.08950.3445-0.4796-0.28070.2397-0.7577-0.2998-0.12160.47890.11310.4663-0.01740.11660.521-0.00360.4791-10.5231-20.7494-11.4248
46.1827-0.1089-1.62538.0624-1.91744.34-0.31530.186-0.3876-0.47980.1528-0.3279-0.06910.15990.16060.2966-0.01930.09430.5165-0.09240.3968-6.1176-14.9201-15.9403
57.2808-3.753-0.29481.96190.29220.3416-0.39220.1459-1.37870.7461-0.49370.43660.17480.85290.70960.82890.11810.37231.16850.3181.055313.0595-24.3639-12.8435
67.21117.39070.3957.8024-0.65018.09040.1959-0.2914-0.09980.62080.0674-0.95750.17750.0962-0.27440.62980.21650.08610.4898-0.11770.73445.5423-43.934-22.2968
74.59764.6703-3.31967.3682-1.09978.477-0.1022-0.9225-0.21180.2388-0.29550.64271.35540.65660.30651.02360.06470.33730.40350.05240.739-4.6371-47.5182-18.0662
82.4912.4546-0.17999.2929-4.00165.2909-0.01430.2693-0.97210.4836-0.32160.9520.86720.29630.05170.88490.19760.15240.46660.09620.6971-14.8898-43.4037-21.561
93.0738-0.7623.38431.2753-1.33753.97561.80741.34521.6688-0.4454-1.25590.0550.83110.6262-0.35150.79040.1380.32980.5110.03810.6831-9.5677-30.8161-28.0815
104.6391-2.88931.75944.16521.35793.4329-0.6187-0.74621.60840.03640.667-0.6964-0.2728-0.1534-0.43470.66490.14120.14260.465-0.02750.5961-2.8361-33.6953-18.4551
118.4068-4.0292-4.07018.6445.77094.10350.97121.6017-0.8592-0.981-1.1950.1285-0.0804-0.28020.06560.73120.14110.07090.4154-0.04090.4702-5.5538-43.8374-26.4868
126.0813-4.2799-0.99444.22173.05214.9051-0.0749-0.41771.35880.21980.42010.5711-1.84260.315-0.35670.72510.06740.23230.5420.08220.897519.3083-51.1648-26.7485
135.22452.36166.10187.535-0.5058.767-1.5438-0.0878-0.88221.03810.29741.5764-0.6544-0.99760.95580.82940.25830.19050.7214-0.12910.723411.2233-52.3663-29.8579
144.27811.87643.58843.12870.71083.48770.08622.7283-0.1036-0.85250.02822.2353-1.0970.2804-0.12950.91590.2044-0.12160.7971-0.1560.794514.5808-57.0521-38.4225
158.80710.73211.02978.40843.40458.06570.1452-0.30620.22590.21470.02630.5238-0.4514-0.9616-0.08010.28060.03360.05090.4250.07070.271522.6637-67.8675-34.2582
166.9827-4.5299-1.90537.74142.22836.94360.1263-0.8031-0.00920.6936-0.13380.7112-0.5240.1120.00720.42610.08460.0930.3631-0.04730.396920.6033-60.8749-31.0949
176.9627-4.40750.64653.18610.66235.17280.29281.2753-1.3266-0.4238-0.34021.37550.17110.66-0.13440.7750.205-0.11711.055-0.34851.0833-0.7856-52.9451-35.386
184.86914.44121.41856.39945.81989.21250.1638-0.04820.7435-0.8555-0.45670.607-0.6186-0.49520.2320.54940.05460.19290.63850.08310.783119.9128-10.4482-25.3482
195.8983-0.08015.15169.2476-2.45727.224-0.34740.2237-1.236-0.2544-0.11691.67131.0998-1.41660.28010.431-0.06330.16570.76990.07310.829215.5829-18.0145-24.2258
203.8957-4.17973.84068.215-4.60733.85950.15771.1053-1.2871-1.2916-0.56910.65531.4331-0.59920.26770.5643-0.10540.10360.651-0.16640.768122.9439-24.1702-29.1595
216.41933.87781.83595.72510.09627.7799-0.1212-0.6722-0.5361-0.5388-0.5393-0.2710.5842-0.43190.56710.42770.04560.10540.4669-0.09320.473136.1624-23.8381-23.023
223.6945-1.0978-4.77932.8298-0.38988.52150.7138-1.64140.9090.54730.08310.5779-1.95110.8014-0.05070.6249-0.06320.21710.7347-0.17330.642333.2732-11.4748-22.4414
239.4427-3.02460.16966.6576-0.95474.46790.55391.57970.2971-0.5877-0.4024-0.1684-0.11080.0455-0.07680.50520.0330.22160.6572-0.03470.530931.3722-12.0604-29.6342
248.8142-1.9485-2.13046.8349-1.30526.396-0.6574-1.3972-1.04120.31140.2480.68350.4088-0.49730.27320.4101-0.04870.16530.51080.0120.525826.4869-20.4621-20.5167
254.2885-5.4485-1.94318.38174.03483.29360.1633-0.2885-0.940.5297-0.1876-0.04951.869-0.1148-0.09541.00060.0860.18430.5027-0.13440.725943.0748-33.1099-42.1209
269.59883.37813.40922.856-1.53456.4979-0.02551.425-1.0003-2.0666-0.2626-0.1072-1.1514-1.35190.23710.91780.08630.02540.66-0.14830.468737.2122-24.298-45.5882
278.03562.8548-1.04276.08613.93523.7321-0.20861.29460.2766-1.0122-0.31270.3622-0.3201-0.15480.73580.52840.07060.05060.52680.0130.284340.7383-15.1772-33.3045
287.21470.6121-0.84064.2558-1.24147.9223-0.32860.0674-0.9832-1.1065-0.4265-0.94650.96430.34010.64660.71370.09170.16380.4182-0.0390.315341.9075-23.2866-35.076
291.4073-1.50451.71572.441-1.71252.45490.0502-0.233-0.0958-0.26210.31720.6038-1.8860.5945-0.48351.24680.2810.20440.8858-0.05910.66238.711-19.7302-55.3625
306.81690.03571.56745.1505-0.80545.94410.0628-0.5193-0.5790.3530.040.2710.45520.1435-0.16490.8373-0.11290.29890.6640.07570.607445.8174-39.862-2.4227
313.9907-3.71083.42114.5752-3.53573.52150.5733-2.96431.5876-0.40351.5542-0.28430.53590.5196-2.57480.8706-0.09850.26441.40690.25781.076661.3004-32.2555-6.7372
329.4280.30333.44815.31183.32586.1796-1.40250.54120.2034-0.78330.07450.10950.4251-0.74130.69020.4499-0.00210.25130.42360.03040.564449.7526-28.4765-17.5338
336.74096.7412-1.68668.3538-4.35688.089-0.1009-0.35220.0049-0.0375-0.61590.93580.0591-0.35890.46910.35350.14320.15180.46910.00370.4547.0221-34.8308-11.5428
343.9040.5953.6070.6803-0.66159.4149-0.6709-0.3983-1.29730.05270.7901-1.4312-0.9860.58380.3340.8712-0.05660.20251.1065-0.04710.792651.8747-34.35097.6559
357.33713.6949-4.61999.3468-2.21782.910.0450.1197-0.82130.6084-0.44610.1927-0.62990.50930.58171.0869-0.01880.23560.5125-0.03860.452143.5852-38.9572-57.3976
361.46410.05350.00688.7876-5.42263.4497-0.1953-1.20030.89072.08960.015-0.5904-1.11310.1971-0.21490.91860.0390.20690.8345-0.22860.504846.2718-30.3741-57.2977
372.12630.47190.61012.2942-3.71447.55220.3382-1.18281.1498-1.6336-0.0873-1.1007-0.53961.8094-0.13170.8124-0.07060.18970.7481-0.26170.831651.3949-29.0649-66.3062
386.4459-0.71663.1761.969-2.426.2567-0.94321.27570.3731-0.53960.2854-0.536-0.9159-0.4160.69860.6894-0.10570.10920.6053-0.01690.526449.3325-25.4776-75.9942
393.3159-1.6996-1.60693.8787-4.34249.6053-0.3240.1036-0.12440.2462-0.12520.12830.4216-2.12910.07030.5807-0.03640.15810.6671-0.14880.522738.0018-36.8493-77.05
408.916-4.15690.36157.18160.31443.4107-0.6046-0.3162-0.1290.99070.23750.7633-0.1057-0.04530.32360.625-0.07110.16910.5814-0.20640.43743.2385-34.7836-68.705
415.78421.2141.45158.47592.94788.68610.05180.18780.3243-1.2217-0.0020.3972-0.2843-0.58240.04640.8315-0.0310.22570.73830.0870.53836.6896-41.888410.7001
424.03480.8923.45627.9593-1.3883.62650.4316-0.17561.49210.5301-0.6597-0.1018-1.43730.01540.10361.1563-0.09530.33880.6030.07250.881636.262-32.908219.9317
434.9244-3.23394.20012.154-2.72533.65720.00730.35740.04840.1353-1.0457-0.5644-0.30971.18531.25290.74930.02480.15620.7720.12180.705940.825-40.44431.4533
442.91.69790.41947.20260.94985.1181-0.42610.7748-0.76110.20380.2564-0.43010.53680.04930.31840.661-0.03920.26990.5750.08810.571938.1457-44.069822.3766
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1chain 'A' and (resid 419 through 429 )
2X-RAY DIFFRACTION2chain 'A' and (resid 430 through 449 )
3X-RAY DIFFRACTION3chain 'A' and (resid 450 through 464 )
4X-RAY DIFFRACTION4chain 'A' and (resid 465 through 484 )
5X-RAY DIFFRACTION5chain 'A' and (resid 485 through 498 )
6X-RAY DIFFRACTION6chain 'B' and (resid 419 through 438 )
7X-RAY DIFFRACTION7chain 'B' and (resid 439 through 449 )
8X-RAY DIFFRACTION8chain 'B' and (resid 450 through 459 )
9X-RAY DIFFRACTION9chain 'B' and (resid 460 through 464 )
10X-RAY DIFFRACTION10chain 'B' and (resid 465 through 473 )
11X-RAY DIFFRACTION11chain 'B' and (resid 474 through 488 )
12X-RAY DIFFRACTION12chain 'C' and (resid 420 through 429 )
13X-RAY DIFFRACTION13chain 'C' and (resid 430 through 438 )
14X-RAY DIFFRACTION14chain 'C' and (resid 439 through 450 )
15X-RAY DIFFRACTION15chain 'C' and (resid 451 through 465 )
16X-RAY DIFFRACTION16chain 'C' and (resid 466 through 487 )
17X-RAY DIFFRACTION17chain 'C' and (resid 488 through 500 )
18X-RAY DIFFRACTION18chain 'D' and (resid 420 through 429 )
19X-RAY DIFFRACTION19chain 'D' and (resid 430 through 438 )
20X-RAY DIFFRACTION20chain 'D' and (resid 439 through 451 )
21X-RAY DIFFRACTION21chain 'D' and (resid 452 through 461 )
22X-RAY DIFFRACTION22chain 'D' and (resid 462 through 466 )
23X-RAY DIFFRACTION23chain 'D' and (resid 467 through 473 )
24X-RAY DIFFRACTION24chain 'D' and (resid 474 through 487 )
25X-RAY DIFFRACTION25chain 'E' and (resid 419 through 429 )
26X-RAY DIFFRACTION26chain 'E' and (resid 430 through 449 )
27X-RAY DIFFRACTION27chain 'E' and (resid 450 through 464 )
28X-RAY DIFFRACTION28chain 'E' and (resid 465 through 483 )
29X-RAY DIFFRACTION29chain 'E' and (resid 484 through 497 )
30X-RAY DIFFRACTION30chain 'F' and (resid 418 through 449 )
31X-RAY DIFFRACTION31chain 'F' and (resid 450 through 454 )
32X-RAY DIFFRACTION32chain 'F' and (resid 455 through 464 )
33X-RAY DIFFRACTION33chain 'F' and (resid 465 through 484 )
34X-RAY DIFFRACTION34chain 'F' and (resid 485 through 495 )
35X-RAY DIFFRACTION35chain 'G' and (resid 418 through 429 )
36X-RAY DIFFRACTION36chain 'G' and (resid 430 through 438 )
37X-RAY DIFFRACTION37chain 'G' and (resid 439 through 450 )
38X-RAY DIFFRACTION38chain 'G' and (resid 451 through 455 )
39X-RAY DIFFRACTION39chain 'G' and (resid 456 through 465 )
40X-RAY DIFFRACTION40chain 'G' and (resid 466 through 487 )
41X-RAY DIFFRACTION41chain 'H' and (resid 419 through 438 )
42X-RAY DIFFRACTION42chain 'H' and (resid 439 through 451 )
43X-RAY DIFFRACTION43chain 'H' and (resid 452 through 461 )
44X-RAY DIFFRACTION44chain 'H' and (resid 462 through 487 )

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