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Open data
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Basic information
Entry | Database: PDB / ID: 2mb2 | ||||||||||||||||||||
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Title | parallel-stranded G-quadruplex in DNA poly-G stretches | ||||||||||||||||||||
![]() | DNA_(5'-D(*![]() DNA / G-tracts / G-quadruplex / propeller structure / poly-G stretches | Function / homology | DNA / DNA (> 10) | ![]() Method | SOLUTION NMR / DGSA-distance geometry simulated annealing, simulated annealing | Model details | lowest energy, model1 | ![]() Sengar, A. / Heddi, B. / Phan, A.T. | ![]() ![]() Title: Formation of g-quadruplexes in poly-g sequences: structure of a propeller-type parallel-stranded g-quadruplex formed by a g15 stretch. Authors: Sengar, A. / Heddi, B. / Phan, A.T. History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 122.5 KB | Display | ![]() |
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PDB format | ![]() | 103.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
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-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Similar structure data | |
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: DNA chain | Mass: 5805.709 Da / Num. of mol.: 1 / Source method: obtained synthetically |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||
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NMR experiment |
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Sample preparation
Details | Contents: 0.1-2 mM DNA (5'-D(*TP*TP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*T)-3'), 90% H2O/10% D2O Solvent system: 90% H2O/10% D2O |
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Sample | Units: mM Component: DNA (5'-D(*TP*TP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*T)-3')-1 Conc. range: 0.1-2 |
Sample conditions | Ionic strength: 10 / pH: 7 / Pressure: ambient / Temperature: 310 K |
-NMR measurement
NMR spectrometer |
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Processing
NMR software |
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Refinement | Method: DGSA-distance geometry simulated annealing, simulated annealing Software ordinal: 1 | ||||||||||||||||
NMR constraints | NA chi-angle constraints total count: 12 / NOE constraints total: 305 / NOE intraresidue total count: 155 / NOE medium range total count: 48 / NOE sequential total count: 91 / Hydrogen bond constraints total count: 48 | ||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 10 |