+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 2m6i | ||||||
---|---|---|---|---|---|---|---|
タイトル | Putative pentameric open-channel structure of full-length transmembrane domains of human glycine receptor alpha1 subunit | ||||||
要素 | Full-Length Transmembrane Domains of Human Glycine Receptor alpha1 Subunit | ||||||
キーワード | MEMBRANE PROTEIN (膜タンパク質) / glycine receptor / anion channel (イオンチャネル) / transmembrane domain (膜貫通型ドメイン) / pentamer structure | ||||||
機能・相同性 | 機能・相同性情報 taurine binding / response to alcohol / negative regulation of transmission of nerve impulse / Neurotransmitter receptors and postsynaptic signal transmission / positive regulation of acrosome reaction / 先体反応 / neuromuscular process controlling posture / inhibitory synapse / righting reflex / regulation of respiratory gaseous exchange by nervous system process ...taurine binding / response to alcohol / negative regulation of transmission of nerve impulse / Neurotransmitter receptors and postsynaptic signal transmission / positive regulation of acrosome reaction / 先体反応 / neuromuscular process controlling posture / inhibitory synapse / righting reflex / regulation of respiratory gaseous exchange by nervous system process / extracellularly glycine-gated chloride channel activity / synaptic transmission, glycinergic / glycinergic synapse / inhibitory postsynaptic potential / cellular response to ethanol / adult walking behavior / chloride transport / cellular response to zinc ion / glycine binding / startle response / chloride channel complex / neuropeptide signaling pathway / neuronal action potential / response to amino acid / monoatomic ion transport / chloride transmembrane transport / ligand-gated monoatomic ion channel activity involved in regulation of presynaptic membrane potential / 視覚 / regulation of membrane potential / transmitter-gated monoatomic ion channel activity involved in regulation of postsynaptic membrane potential / muscle contraction / cellular response to amino acid stimulus / transmembrane signaling receptor activity / postsynaptic membrane / perikaryon / neuron projection / external side of plasma membrane / intracellular membrane-bounded organelle / neuronal cell body / シナプス / 樹状突起 / zinc ion binding / 生体膜 / identical protein binding / 細胞膜 類似検索 - 分子機能 | ||||||
生物種 | Homo sapiens (ヒト) | ||||||
手法 | 溶液NMR / simulated annealing | ||||||
データ登録者 | Mowrey, D. / Cui, T. / Jia, Y. / Ma, D. / Makhov, A.M. / Zhang, P. / Tang, P. / Xu, Y. | ||||||
引用 | ジャーナル: Structure / 年: 2013 タイトル: Open-Channel Structures of the Human Glycine Receptor alpha 1 Full-Length Transmembrane Domain. 著者: Mowrey, D.D. / Cui, T. / Jia, Y. / Ma, D. / Makhov, A.M. / Zhang, P. / Tang, P. / Xu, Y. | ||||||
履歴 |
|
-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
---|
-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 2m6i.cif.gz | 4 MB | 表示 | PDBx/mmCIF形式 |
---|---|---|---|---|
PDB形式 | pdb2m6i.ent.gz | 3.5 MB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 2m6i.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/m6/2m6i ftp://data.pdbj.org/pub/pdb/validation_reports/m6/2m6i | HTTPS FTP |
---|
-関連構造データ
-リンク
-集合体
登録構造単位 |
| |||||||||
---|---|---|---|---|---|---|---|---|---|---|
1 |
| |||||||||
NMR アンサンブル |
|
-要素
#1: タンパク質 | 分子量: 17320.320 Da / 分子数: 5 / 由来タイプ: 組換発現 / 由来: (組換発現) Homo sapiens (ヒト) / プラスミド: pET-31b(+) / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: P23415*PLUS |
---|
-実験情報
-実験
実験 | 手法: 溶液NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
NMR実験 |
|
-試料調製
詳細 | 内容: protein, 95% H2O/5% D2O / 溶媒系: 95% H2O/5% D2O |
---|---|
試料状態 | pH: 5.8 / 圧: ambient / 温度: 313 K |
-NMR測定
NMRスペクトロメーター |
|
---|
-解析
NMR software | 名称: CYANA / バージョン: 3 / 開発者: Guntert, P. et al. / 分類: 精密化 |
---|---|
精密化 | 手法: simulated annealing / ソフトェア番号: 1 |
NMR constraints | NOE constraints total: 5070 / NOE intraresidue total count: 1605 / NOE long range total count: 105 / NOE medium range total count: 1620 / NOE sequential total count: 1740 / Hydrogen bond constraints total count: 1060 / Protein phi angle constraints total count: 385 / Protein psi angle constraints total count: 385 |
代表構造 | 選択基準: closest to the average |
NMRアンサンブル | コンフォーマー選択の基準: target function / 計算したコンフォーマーの数: 100 / 登録したコンフォーマーの数: 15 / Maximum torsion angle constraint violation: 3.32 ° / Maximum upper distance constraint violation: 0.63 Å |
NMR ensemble rms | Distance rms dev: 0.0157 Å / Distance rms dev error: 0.001 Å |