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- PDB-2m3t: Solution-state NMR structure of wild-type human gamma(S)-crystallin -
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Open data
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Basic information
Entry | Database: PDB / ID: 2m3t | ||||||
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Title | Solution-state NMR structure of wild-type human gamma(S)-crystallin | ||||||
![]() | Beta-crystallin S | ||||||
![]() | STRUCTURAL PROTEIN / gamma-S / eye lens / aggregation / crystallin / cataract / CRYGS | ||||||
Function / homology | ![]() structural constituent of eye lens / lens development in camera-type eye / visual perception / morphogenesis of an epithelium Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Model details | minimized average structure, model 1 | ||||||
Model type details | minimized average | ||||||
![]() | Brubaker, W.D. / Martin, R.W. | ||||||
![]() | ![]() Title: Preferential and Specific Binding of Human alpha B-Crystallin to a Cataract-Related Variant of gamma S-Crystallin. Authors: Kingsley, C.N. / Brubaker, W.D. / Markovic, S. / Diehl, A. / Brindley, A.J. / Oschkinat, H. / Martin, R.W. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 1.1 MB | Display | ![]() |
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PDB format | ![]() | 978.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 408.8 KB | Display | ![]() |
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Full document | ![]() | 617.1 KB | Display | |
Data in XML | ![]() | 97.6 KB | Display | |
Data in CIF | ![]() | 127.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: Protein | Mass: 20959.633 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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Sample preparation
Details |
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Sample |
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Sample conditions |
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-NMR measurement
NMR spectrometer | Type: Varian INOVA / Manufacturer: Varian / Model: INOVA / Field strength: 800 MHz |
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Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | ||||||||||||||||||||||||||||||||||||
NMR constraints | NOE constraints total: 7444 / NOE intraresidue total count: 1547 / NOE long range total count: 3052 / NOE medium range total count: 1286 / NOE sequential total count: 1559 | ||||||||||||||||||||||||||||||||||||
NMR representative | Selection criteria: minimized average structure | ||||||||||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 200 / Conformers submitted total number: 21 |