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- PDB-2m1f: NMR Structure of Antiamoebin I (peptaibol antibiotic) bound to DM... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2m1f | ||||||
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Title | NMR Structure of Antiamoebin I (peptaibol antibiotic) bound to DMPC/DHPC bicelles | ||||||
![]() | Antiamoebin I | ||||||
![]() | ANTIBIOTIC / membrane-active / peptaibol | ||||||
Function / homology | Antiamoebin 1 / : ![]() | ||||||
Biological species | ![]() | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Model details | fewest violations, model1 | ||||||
![]() | Shenkarev, Z.O. / Paramonov, A.S. / Gizatullina, A.K. | ||||||
![]() | ![]() Title: Peptaibol antiamoebin I: spatial structure, backbone dynamics, interaction with bicelles and lipid-protein nanodiscs, and pore formation in context of barrel-stave model. Authors: Shenkarev, Z.O. / Paramonov, A.S. / Lyukmanova, E.N. / Gizatullina, A.K. / Zhuravleva, A.V. / Tagaev, A.A. / Yakimenko, Z.A. / Telezhinskaya, I.N. / Kirpichnikov, M.P. / Ovchinnikova, T.V. / Arseniev, A.S. | ||||||
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 102.5 KB | Display | ![]() |
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PDB format | ![]() | 82.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 365.9 KB | Display | ![]() |
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Full document | ![]() | 383 KB | Display | |
Data in XML | ![]() | 11.4 KB | Display | |
Data in CIF | ![]() | 16.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Similar structure data | |
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||
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NMR experiment |
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Sample preparation
Details | Contents: 120 mM DHPC, 30 mM DMPC, 7.5 mM acetic acid, 2.5 mM sodium acetate, 1 mM [U-100% 13C; U-100% 15N] ANTIAMOEBIN I, 90% H2O/10% D2O Solvent system: 90% H2O/10% D2O | ||||||||||||||||||||||||
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Sample |
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Sample conditions | pH: 5.1 / Pressure: ambient / Temperature: 313 K |
-NMR measurement
NMR spectrometer | Type: Bruker Avance / Manufacturer: Bruker / Model: Avance / Field strength: 600 MHz |
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Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | |||||||||||||||||||||
NMR representative | Selection criteria: fewest violations | |||||||||||||||||||||
NMR ensemble | Conformer selection criteria: target function / Conformers calculated total number: 50 / Conformers submitted total number: 20 |