+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 2lqw | ||||||
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タイトル | Solution structure of phosphorylated CRKL | ||||||
要素 | Crk-like protein | ||||||
キーワード | SIGNALING PROTEIN / SH2 / SH3 / v-crk sarcoma virus CT10 / oncogene homolog / (avian)-like / pCRKL | ||||||
機能・相同性 | 機能・相同性情報 positive regulation of glial cell migration / chordate pharynx development / extrinsic component of postsynaptic membrane / helper T cell diapedesis / cerebellar neuron development / postsynaptic specialization assembly / urogenital system development / parathyroid gland development / regulation of T cell migration / reelin-mediated signaling pathway ...positive regulation of glial cell migration / chordate pharynx development / extrinsic component of postsynaptic membrane / helper T cell diapedesis / cerebellar neuron development / postsynaptic specialization assembly / urogenital system development / parathyroid gland development / regulation of T cell migration / reelin-mediated signaling pathway / endothelin receptor signaling pathway / regulation of dendrite development / positive regulation of skeletal muscle acetylcholine-gated channel clustering / cranial skeletal system development / B cell apoptotic process / MET receptor recycling / cellular response to interleukin-7 / acetylcholine receptor signaling pathway / MET activates RAP1 and RAC1 / anterior/posterior pattern specification / Frs2-mediated activation / establishment of cell polarity / blood vessel development / dendrite development / outflow tract morphogenesis / positive regulation of Rac protein signal transduction / regulation of cell adhesion mediated by integrin / single fertilization / retinoic acid receptor signaling pathway / fibroblast growth factor receptor signaling pathway / signaling adaptor activity / Erythropoietin activates RAS / positive regulation of substrate adhesion-dependent cell spreading / JNK cascade / cellular response to transforming growth factor beta stimulus / phosphotyrosine residue binding / cell chemotaxis / Downstream signal transduction / thymus development / negative regulation of protein phosphorylation / Regulation of signaling by CBL / regulation of cell growth / hippocampus development / neuron migration / neuromuscular junction / lipid metabolic process / receptor tyrosine kinase binding / cerebral cortex development / male gonad development / 遊走 / cellular response to xenobiotic stimulus / T cell receptor signaling pathway / 精子形成 / RNA polymerase II-specific DNA-binding transcription factor binding / Ras protein signal transduction / positive regulation of ERK1 and ERK2 cascade / intracellular signal transduction / cadherin binding / positive regulation of protein phosphorylation / negative regulation of gene expression / positive regulation of cell population proliferation / シグナル伝達 / protein-containing complex / RNA binding / 核質 / identical protein binding / 細胞質基質 類似検索 - 分子機能 | ||||||
生物種 | Homo sapiens (ヒト) | ||||||
手法 | 溶液NMR / simulated annealing | ||||||
Model details | closest to the average, model 1 | ||||||
データ登録者 | Jankowski, W. / Saleh, T. / Kalodimos, C. | ||||||
引用 | ジャーナル: Nat.Chem.Biol. / 年: 2012 タイトル: Domain organization differences explain Bcr-Abl's preference for CrkL over CrkII. 著者: Jankowski, W. / Saleh, T. / Pai, M.T. / Sriram, G. / Birge, R.B. / Kalodimos, C.G. | ||||||
履歴 |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 2lqw.cif.gz | 1.8 MB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb2lqw.ent.gz | 1.5 MB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 2lqw.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/lq/2lqw ftp://data.pdbj.org/pub/pdb/validation_reports/lq/2lqw | HTTPS FTP |
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-関連構造データ
-リンク
-集合体
登録構造単位 |
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1 |
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NMR アンサンブル |
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-要素
#1: タンパク質 | 分子量: 33896.887 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Homo sapiens (ヒト) / 遺伝子: CRKL / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: P46109 |
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-実験情報
-実験
実験 | 手法: 溶液NMR |
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NMR実験 | タイプ: 2D 1H-15N HSQC |
-試料調製
詳細 | 内容: 0.3-0.8 mM [U-100% 15N] potassium phosphate, 0.3-0.8 mM [U-100% 13C; U-100% 15N] potassium chloride, 0.3-0.8 mM [U-13C; U-15N; U-2H] beta-mercaptoethanol, 90% H2O/10% D2O 溶媒系: 90% H2O/10% D2O | ||||||||||||||||||||
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試料 |
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試料状態 | イオン強度: 0.15 / pH: 6.8 / 圧: ambient / 温度: 305 K |
-NMR測定
NMRスペクトロメーター |
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-解析
NMR software | 名称: CNS / 開発者: Brunger A. T. et.al. / 分類: 精密化 |
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精密化 | 手法: simulated annealing / ソフトェア番号: 1 |
代表構造 | 選択基準: closest to the average |
NMRアンサンブル | コンフォーマー選択の基準: structures with the lowest energy 計算したコンフォーマーの数: 400 / 登録したコンフォーマーの数: 20 |