+Open data
-Basic information
Entry | Database: PDB / ID: 2lom | ||||||
---|---|---|---|---|---|---|---|
Title | Backbone structure of human membrane protein HIGD1A | ||||||
Components | HIG1 domain family member 1A | ||||||
Keywords | MEMBRANE PROTEIN / Helical bundle | ||||||
Function / homology | Function and homology information Complex IV assembly / mitochondrial respirasome assembly / Regulation of gene expression by Hypoxia-inducible Factor / : / mitochondrial inner membrane / negative regulation of apoptotic process / protein-containing complex / mitochondrion / nucleoplasm Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | SOLUTION NMR / torsion angle dynamics | ||||||
Model details | fewest violations, model 1 | ||||||
Authors | Blain, K. / Klammt, C. / Maslennikov, I. / Kwiatkowski, W. / Choe, S. | ||||||
Citation | Journal: Nat.Methods / Year: 2012 Title: Facile backbone structure determination of human membrane proteins by NMR spectroscopy. Authors: Klammt, C. / Maslennikov, I. / Bayrhuber, M. / Eichmann, C. / Vajpai, N. / Chiu, E.J. / Blain, K.Y. / Esquivies, L. / Kwon, J.H. / Balana, B. / Pieper, U. / Sali, A. / Slesinger, P.A. / ...Authors: Klammt, C. / Maslennikov, I. / Bayrhuber, M. / Eichmann, C. / Vajpai, N. / Chiu, E.J. / Blain, K.Y. / Esquivies, L. / Kwon, J.H. / Balana, B. / Pieper, U. / Sali, A. / Slesinger, P.A. / Kwiatkowski, W. / Riek, R. / Choe, S. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 2lom.cif.gz | 562 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb2lom.ent.gz | 468.9 KB | Display | PDB format |
PDBx/mmJSON format | 2lom.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2lom_validation.pdf.gz | 480.1 KB | Display | wwPDB validaton report |
---|---|---|---|---|
Full document | 2lom_full_validation.pdf.gz | 944.5 KB | Display | |
Data in XML | 2lom_validation.xml.gz | 121.7 KB | Display | |
Data in CIF | 2lom_validation.cif.gz | 91.4 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/lo/2lom ftp://data.pdbj.org/pub/pdb/validation_reports/lo/2lom | HTTPS FTP |
-Related structure data
Related structure data | 2lonC 2looC 2lopC 2loqC 2lorC 2losC C: citing same article (ref.) |
---|---|
Similar structure data | |
Other databases |
-Links
-Assembly
Deposited unit |
| |||||||||
---|---|---|---|---|---|---|---|---|---|---|
1 |
| |||||||||
NMR ensembles |
|
-Components
#1: Protein | Mass: 10156.917 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: HIGD1A, HIG1, HSPC010 / Production host: CELL-FREE SYNTHESIS (others) / References: UniProt: Q9Y241 |
---|
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
NMR experiment |
|
-Sample preparation
Details |
| |||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Sample |
| |||||||||||||||||||||||||||||||||||||||
Sample conditions | Ionic strength: 40 / pH: 6 / Pressure: ambient / Temperature: 310 K |
-NMR measurement
NMR spectrometer | Type: Bruker DRX / Manufacturer: Bruker / Model: DRX / Field strength: 700 MHz |
---|
-Processing
NMR software |
| ||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method: torsion angle dynamics / Software ordinal: 1 | ||||||||||||||||||||||||||||||||
NMR representative | Selection criteria: fewest violations | ||||||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: target function / Conformers calculated total number: 200 / Conformers submitted total number: 20 |