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- PDB-2loq: Backbone structure of human membrane protein FAM14B (Interferon a... -

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Basic information

Entry
Database: PDB / ID: 2loq
TitleBackbone structure of human membrane protein FAM14B (Interferon alpha-inducible protein 27-like protein 1)
ComponentsInterferon alpha-inducible protein 27-like protein 1
KeywordsMEMBRANE PROTEIN / Helical bundle
Function / homology
Function and homology information


intrinsic apoptotic signaling pathway / mitochondrial membrane / molecular adaptor activity / apoptotic process
Similarity search - Function
Serum Albumin; Chain A, Domain 1 / Serum Albumin; Chain A, Domain 1 - #10 / Interferon alpha-inducible protein IFI6/IFI27-like / IFI6/IFI27-like domain superfamily / Interferon-induced 6-16 family / Helix non-globular / Special
Similarity search - Domain/homology
Interferon alpha-inducible protein 27-like protein 1
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodSOLUTION NMR / torsion angle dynamics
Model detailsfewest violations, model 1
AuthorsKlammt, C. / Chui, E.J. / Maslennikov, I. / Kwiatkowski, W. / Choe, S.
CitationJournal: Nat.Methods / Year: 2012
Title: Facile backbone structure determination of human membrane proteins by NMR spectroscopy.
Authors: Klammt, C. / Maslennikov, I. / Bayrhuber, M. / Eichmann, C. / Vajpai, N. / Chiu, E.J. / Blain, K.Y. / Esquivies, L. / Kwon, J.H. / Balana, B. / Pieper, U. / Sali, A. / Slesinger, P.A. / ...Authors: Klammt, C. / Maslennikov, I. / Bayrhuber, M. / Eichmann, C. / Vajpai, N. / Chiu, E.J. / Blain, K.Y. / Esquivies, L. / Kwon, J.H. / Balana, B. / Pieper, U. / Sali, A. / Slesinger, P.A. / Kwiatkowski, W. / Riek, R. / Choe, S.
History
DepositionJan 26, 2012Deposition site: BMRB / Processing site: RCSB
Revision 1.0May 23, 2012Provider: repository / Type: Initial release
Revision 1.1Aug 15, 2012Group: Database references
Revision 1.2Jun 14, 2023Group: Data collection / Database references / Other
Category: database_2 / pdbx_database_status ...database_2 / pdbx_database_status / pdbx_nmr_software / struct_ref_seq_dif
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_database_status.status_code_nmr_data / _pdbx_nmr_software.name / _struct_ref_seq_dif.details
Revision 1.3May 15, 2024Group: Data collection / Database references / Category: chem_comp_atom / chem_comp_bond / database_2 / Item: _database_2.pdbx_DOI

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: Interferon alpha-inducible protein 27-like protein 1


Theoretical massNumber of molelcules
Total (without water)10,5631
Polymers10,5631
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
NMR ensembles
DataCriteria
Number of conformers (submitted / calculated)20 / 200target function
RepresentativeModel #1fewest violations

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Components

#1: Protein Interferon alpha-inducible protein 27-like protein 1 / Interferon-stimulated gene 12c protein / ISG12(c)


Mass: 10563.238 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: IFI27L1, FAM14B / Production host: CELL-FREE SYNTHESIS (others) / References: UniProt: Q96BM0

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Experimental details

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Experiment

ExperimentMethod: SOLUTION NMR
NMR experiment
Conditions-IDExperiment-IDSolution-IDType
1122D 1H-15N HSQC
1222D HNCO
1333D HNCO
1433D HNCA
1533D HN(CA)CB
1612D 1H-15N HSQC
1713D 1H-15N NOESY
1843D 1H-15N NOESY
1933D 13C-15N HSQC-NOESY-HSQC

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Sample preparation

Details
Solution-IDContentsSolvent system
115N-FAM14B, 20 mM MES-Bis-TRIS, 3 % LMPG, 0.5 mM DSS, 95% H2O/5% D2O95% H2O/5% D2O
2selectively labeled 13C(1),15N-FAM14B, 20 mM MES-Bis-TRIS, 3 % LMPG, 0.5 mM DSS, 95% H2O/5% D2O95% H2O/5% D2O
313C-15N-FAM14B, 20 mM MES-Bis-TRIS, 3 % LMPG, 0.5 mM DSS, 95% H2O/5% D2O95% H2O/5% D2O
415N,2H-FAM14B, 20 mM MES-Bis-TRIS, 3 % LMPG, 0.5 mM DSS, 95% H2O/5% D2O95% H2O/5% D2O
Sample
Conc. (mg/ml)ComponentSolution-ID
20 mMMES-Bis-TRIS-11
3 %LMPG-21
0.5 mMDSS-31
20 mMMES-Bis-TRIS-42
3 %LMPG-52
0.5 mMDSS-62
20 mMMES-Bis-TRIS-73
3 %LMPG-83
0.5 mMDSS-93
20 mMMES-Bis-TRIS-104
3 %LMPG-114
0.5 mMDSS-124
20 mMMES-Bis-TRIS-135
3 %LMPG-145
0.5 mMDSS-155
20 mMMES-Bis-TRIS-166
3 %LMPG-176
0.5 mMDSS-186
20 mMMES-Bis-TRIS-197
3 %LMPG-207
0.5 mMDSS-217
Sample conditionsIonic strength: 60 / pH: 6 / Pressure: ambient / Temperature: 310 K

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NMR measurement

NMR spectrometerType: Bruker DRX / Manufacturer: Bruker / Model: DRX / Field strength: 700 MHz

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Processing

NMR software
NameDeveloperClassification
TopSpinBruker Biospincollection
TopSpinBruker Biospinprocessing
PROSAGuntertprocessing
CARAKeller and Wuthrichchemical shift assignment
CARAKeller and Wuthrichdata analysis
CYANAGuntert, Mumenthaler and Wuthrichstructure solution
MOLMOLKoradi, Billeter and Wuthrichstructure visualization
MOLMOLKoradi, Billeter and Wuthrichstructure analysis
CYANAGuntert, Mumenthaler and Wuthrichrefinement
RefinementMethod: torsion angle dynamics / Software ordinal: 1
NMR representativeSelection criteria: fewest violations
NMR ensembleConformer selection criteria: target function / Conformers calculated total number: 200 / Conformers submitted total number: 20

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