+Open data
-Basic information
Entry | Database: PDB / ID: 2lop | ||||||
---|---|---|---|---|---|---|---|
Title | Backbone structure of human membrane protein TMEM14A | ||||||
Components | Transmembrane protein 14ATransmembrane protein | ||||||
Keywords | MEMBRANE PROTEIN / Helical bundle | ||||||
Function / homology | Function and homology information regulation of heme biosynthetic process / negative regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway / mitochondrial membrane / apoptotic process / endoplasmic reticulum membrane / negative regulation of apoptotic process Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | SOLUTION NMR / torsion angle dynamics | ||||||
Model details | fewest violations, model 1 | ||||||
Authors | Eichmann, C. / Klammt, C. / Maslennikov, I. / Kwiatkowski, W. / Riek, R. / Choe, S. | ||||||
Citation | Journal: Nat.Methods / Year: 2012 Title: Facile backbone structure determination of human membrane proteins by NMR spectroscopy. Authors: Klammt, C. / Maslennikov, I. / Bayrhuber, M. / Eichmann, C. / Vajpai, N. / Chiu, E.J. / Blain, K.Y. / Esquivies, L. / Kwon, J.H. / Balana, B. / Pieper, U. / Sali, A. / Slesinger, P.A. / ...Authors: Klammt, C. / Maslennikov, I. / Bayrhuber, M. / Eichmann, C. / Vajpai, N. / Chiu, E.J. / Blain, K.Y. / Esquivies, L. / Kwon, J.H. / Balana, B. / Pieper, U. / Sali, A. / Slesinger, P.A. / Kwiatkowski, W. / Riek, R. / Choe, S. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 2lop.cif.gz | 611.4 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb2lop.ent.gz | 510.7 KB | Display | PDB format |
PDBx/mmJSON format | 2lop.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/lo/2lop ftp://data.pdbj.org/pub/pdb/validation_reports/lo/2lop | HTTPS FTP |
---|
-Related structure data
Related structure data | 2lomC 2lonC 2looC 2loqC 2lorC 2losC C: citing same article (ref.) |
---|---|
Similar structure data | |
Other databases |
-Links
-Assembly
Deposited unit |
| |||||||||
---|---|---|---|---|---|---|---|---|---|---|
1 |
| |||||||||
NMR ensembles |
|
-Components
#1: Protein | Mass: 11735.306 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: TMEM14A, C6orf73, PTD011 / Production host: CELL-FREE SYNTHESIS (others) / References: UniProt: Q9Y6G1 |
---|
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
NMR experiment |
|
-Sample preparation
Details |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Sample |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sample conditions | Ionic strength: 40 / pH: 6.0 / Pressure: ambient / Temperature: 310 K |
-NMR measurement
NMR spectrometer | Type: Bruker DRX / Manufacturer: Bruker / Model: DRX / Field strength: 700 MHz |
---|
-Processing
NMR software |
| ||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method: torsion angle dynamics / Software ordinal: 1 | ||||||||||||||||||||||||||||||
NMR representative | Selection criteria: fewest violations | ||||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: target function / Conformers calculated total number: 200 / Conformers submitted total number: 20 |