Z-DNA-bindingprotein1 / Tumor stroma and activated macrophage protein DLM-1
Mass: 9041.406 Da / Num. of mol.: 1 / Fragment: DRADA 1 repeat region, residues 6-74 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: C20orf183, DLM1, ZBP1 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3)pMgK / References: UniProt: Q9H171
-
Experimental details
-
Experiment
Experiment
Method: SOLUTION NMR
NMR experiment
Conditions-ID
Experiment-ID
Solution-ID
Type
1
1
1
2D 1H-15N HSQC
1
2
1
2D 1H-13C HSQC aliphatic
1
3
1
3D HNCO
1
4
1
3DCBCA(CO)NH
1
5
1
3D HN(CA)CB
1
6
1
3D 1H-13C arom NOESY
1
7
1
2D 1H-15N HSQC NH2 only
1
8
2
2D 1H-13C HSQC aliphatic
1
9
1
2D 1H-13C HSQC aromatic
1
10
1
3D 1H-15N NOESY
1
11
1
3D 1H-13C NUS-NOESY aliphatic
1
12
1
3D HNCA
1
13
1
3DHN(CO)CA
1
14
1
3DC(CO)NH
1
15
1
3DHBHA(CO)NH
1
16
1
3D (H)CCH-COSY
1
17
1
3D (H)CCH-TOCSY
1
18
1
3D CCH-TOCSY
1
19
1
4D CC-NOESY
-
Sample preparation
Details
Solution-ID
Contents
Solvent system
1
1.0 mM [U-100% 13C; U-100% 15N] N-terminal domain (6-74) of human ZBP1 protein, 20 mM MES, 100 mM sodium chloride, 10 mM DTT, 0.02 % sodium azide, 5 mM calcium chloride, 90% H2O/10% D2O
90% H2O/10% D2O
2
1.00 mM [U-5% 13C; U-100% 15N] N-terminal domain (6-74) of human ZBP1 protein, 20 mM MES, 100 mM sodium chloride, 10 mM DTT, 0.02 % sodium azide, 5 mM calcium chloride, 90% H2O/10% D2O