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- PDB-2lk4: Structural and mechanistic insights into the interaction between ... -

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Basic information

Entry
Database: PDB / ID: 2lk4
TitleStructural and mechanistic insights into the interaction between PAT Pyk2 and Paxillin LD motif
ComponentsProtein-tyrosine kinase 2-beta
KeywordsTRANSFERASE
Function / homology
Function and homology information


regulation of macrophage chemotaxis / response to cation stress / neurotransmitter receptor regulator activity / 3-phosphoinositide-dependent protein kinase binding / positive regulation of B cell chemotaxis / marginal zone B cell differentiation / regulation of ubiquitin-dependent protein catabolic process / blood vessel endothelial cell migration / endothelin receptor signaling pathway / negative regulation of myeloid cell differentiation ...regulation of macrophage chemotaxis / response to cation stress / neurotransmitter receptor regulator activity / 3-phosphoinositide-dependent protein kinase binding / positive regulation of B cell chemotaxis / marginal zone B cell differentiation / regulation of ubiquitin-dependent protein catabolic process / blood vessel endothelial cell migration / endothelin receptor signaling pathway / negative regulation of myeloid cell differentiation / negative regulation of muscle cell apoptotic process / negative regulation of bone mineralization / cortical cytoskeleton organization / regulation of postsynaptic density assembly / activation of Janus kinase activity / positive regulation of ubiquitin-dependent protein catabolic process / apical dendrite / cellular response to fluid shear stress / focal adhesion assembly / regulation of release of sequestered calcium ion into cytosol / signal complex assembly / calcium/calmodulin-dependent protein kinase activity / chemokine-mediated signaling pathway / Interleukin-2 signaling / oocyte maturation / long-term synaptic depression / sprouting angiogenesis / Signal regulatory protein family interactions / NMDA selective glutamate receptor complex / positive regulation of cell-matrix adhesion / positive regulation of DNA biosynthetic process / positive regulation of actin filament polymerization / stress fiber assembly / negative regulation of potassium ion transport / RHOU GTPase cycle / response to immobilization stress / positive regulation of excitatory postsynaptic potential / positive regulation of protein kinase activity / postsynaptic density, intracellular component / glutamate receptor binding / bone resorption / vascular endothelial growth factor receptor signaling pathway / postsynaptic modulation of chemical synaptic transmission / regulation of cell adhesion / glial cell proliferation / cellular defense response / peptidyl-tyrosine autophosphorylation / response to glucose / response to mechanical stimulus / response to cAMP / tumor necrosis factor-mediated signaling pathway / cellular response to retinoic acid / response to hormone / positive regulation of endothelial cell migration / ionotropic glutamate receptor signaling pathway / positive regulation of synaptic transmission, glutamatergic / positive regulation of translation / response to ischemia / integrin-mediated signaling pathway / regulation of actin cytoskeleton organization / positive regulation of JNK cascade / long-term synaptic potentiation / non-membrane spanning protein tyrosine kinase activity / non-specific protein-tyrosine kinase / epidermal growth factor receptor signaling pathway / response to cocaine / peptidyl-tyrosine phosphorylation / Schaffer collateral - CA1 synapse / regulation of synaptic plasticity / response to hydrogen peroxide / positive regulation of neuron projection development / VEGFA-VEGFR2 Pathway / response to calcium ion / neuron projection development / positive regulation of angiogenesis / positive regulation of reactive oxygen species metabolic process / MAPK cascade / positive regulation of nitric oxide biosynthetic process / lamellipodium / regulation of cell shape / presynapse / cell body / growth cone / positive regulation of cytosolic calcium ion concentration / cell cortex / protein tyrosine kinase activity / positive regulation of cell growth / protein-containing complex assembly / protein autophosphorylation / response to ethanol / histone H3Y41 kinase activity / histone H2AXY142 kinase activity / negative regulation of neuron apoptotic process / dendritic spine / adaptive immune response / positive regulation of ERK1 and ERK2 cascade / positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / response to hypoxia / cell surface receptor signaling pathway / cytoskeleton
Similarity search - Function
Nucleotidyltransferases domain 2 / Focal adhesion kinase, targeting (FAT) domain / Focal adhesion kinase, targeting (FAT) domain superfamily / Focal adhesion kinase, N-terminal / FAK1/PYK2, FERM domain C-lobe / Focal adhesion targeting region / FERM N-terminal domain / : / FAK1/PYK2, FERM domain C-lobe / FERM central domain ...Nucleotidyltransferases domain 2 / Focal adhesion kinase, targeting (FAT) domain / Focal adhesion kinase, targeting (FAT) domain superfamily / Focal adhesion kinase, N-terminal / FAK1/PYK2, FERM domain C-lobe / Focal adhesion targeting region / FERM N-terminal domain / : / FAK1/PYK2, FERM domain C-lobe / FERM central domain / FERM/acyl-CoA-binding protein superfamily / FERM central domain / FERM superfamily, second domain / FERM domain / FERM domain profile. / Band 4.1 domain / Band 4.1 homologues / Four Helix Bundle (Hemerythrin (Met), subunit A) / Tyrosine-protein kinase, catalytic domain / Tyrosine kinase, catalytic domain / Tyrosine protein kinases specific active-site signature. / PH-like domain superfamily / Tyrosine-protein kinase, active site / Serine-threonine/tyrosine-protein kinase, catalytic domain / Protein tyrosine and serine/threonine kinase / Ubiquitin-like domain superfamily / Protein kinase, ATP binding site / Protein kinases ATP-binding region signature. / Protein kinase domain profile. / Protein kinase domain / Protein kinase-like domain superfamily / Up-down Bundle / Mainly Alpha
Similarity search - Domain/homology
Protein-tyrosine kinase 2-beta
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodSOLUTION NMR / distance geometry
Model detailsminimized average structure, model 1
Model type detailsminimized average
AuthorsVanarotti, M. / Miller, D. / Guibao, C. / Zheng, J.
CitationJournal: J.Mol.Biol. / Year: 2014
Title: Structural and Mechanistic Insights into the Interaction between Pyk2 and Paxillin LD Motifs.
Authors: Vanarotti, M.S. / Miller, D.J. / Guibao, C.D. / Nourse, A. / Zheng, J.J.
History
DepositionOct 4, 2011Deposition site: BMRB / Processing site: RCSB
Revision 1.0Oct 10, 2012Provider: repository / Type: Initial release
Revision 1.1Sep 24, 2014Group: Database references
Revision 1.2Dec 31, 2014Group: Database references
Revision 1.3May 1, 2024Group: Data collection / Database references
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_nmr_spectrometer / struct_ref_seq_dif
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_nmr_spectrometer.model / _struct_ref_seq_dif.details

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Protein-tyrosine kinase 2-beta


Theoretical massNumber of molelcules
Total (without water)14,8151
Polymers14,8151
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
NMR ensembles
DataCriteria
Number of conformers (submitted / calculated)20 / 100all calculated structures submitted
RepresentativeModel #1minimized average structure

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Components

#1: Protein Protein-tyrosine kinase 2-beta / Calcium-dependent tyrosine kinase / CADTK / Cell adhesion kinase beta / CAK-beta / Focal adhesion ...Calcium-dependent tyrosine kinase / CADTK / Cell adhesion kinase beta / CAK-beta / Focal adhesion kinase 2 / FADK 2 / Proline-rich tyrosine kinase 2 / Related adhesion focal tyrosine kinase / RAFTK


Mass: 14815.045 Da / Num. of mol.: 1
Fragment: Focal adhesion targeting (FAT) region residues 871-1005
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: PTK2B, FAK2, PYK2, RAFTK / Production host: Escherichia coli (E. coli)
References: UniProt: Q14289, non-specific protein-tyrosine kinase

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Experimental details

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Experiment

ExperimentMethod: SOLUTION NMR
NMR experiment
Conditions-IDExperiment-IDSolution-IDType
1113D CBCA(CO)NH
1213D HN(CA)CB
1313D HNCA
1413D (H)CCH-TOCSY
1513D 1H-15N NOESY
1613D 1H-13C NOESY aliphatic
1713D HNHA

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Sample preparation

DetailsContents: 0.5-1 mM [U-100% 13C; U-100% 15N] MES, 90% H2O/10% D2O
Solvent system: 90% H2O/10% D2O
SampleUnits: mM / Component: MES-1 / Isotopic labeling: [U-100% 13C; U-100% 15N] / Conc. range: 0.5-1
Sample conditionsIonic strength: 7 / pH: 6.2 / Pressure: ambient / Temperature: 305 K

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NMR measurement

NMR spectrometer
TypeManufacturerModelField strength (MHz)Spectrometer-ID
Bruker AvanceBrukerAVANCE6001
Bruker AvanceBrukerAVANCE8002

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Processing

NMR software
NameDeveloperClassification
CYANAGuntert, Mumenthaler and Wuthrichstructure solution
CYANAGuntert, Mumenthaler and Wuthrichrefinement
RefinementMethod: distance geometry / Software ordinal: 1
NMR representativeSelection criteria: minimized average structure
NMR ensembleConformer selection criteria: all calculated structures submitted
Conformers calculated total number: 100 / Conformers submitted total number: 20

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